Application | Comment | Organism |
---|---|---|
analysis | construction and evaluation of an ethanol sensor based on the enzyme using direct electron-transfer processes between the polypyrrole entrapped quinohemoprotein alcohol dehydrogenase and a platinum electrode, overview | Gluconobacter sp. |
General Stability | Organism |
---|---|
0.1% Triton X-100 stabilizes the enzyme | Gluconobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, stabilizes the pyrroloquinoline quinone in the active site | Gluconobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter sp. | - |
- |
- |
Gluconobacter sp. DSM 3504 / ATCC 15163 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by anion exchange and hydrophobic interaction chromatography, and dialysis against high-viscosity carboxymethyl cellulose as the absorber | Gluconobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32.2 | - |
purified native enzyme | Gluconobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethanol + acceptor | direct electron-transfer processes between the polypyrrole entrapped quinohemoprotein alcohol dehydrogenase and a platinum electrode take place via the conducting-polymer network, mechanism modelling, overview | Gluconobacter sp. | acetaldehyde + reduced acceptor | - |
? | |
ethanol + acceptor | direct electron-transfer processes between the polypyrrole entrapped quinohemoprotein alcohol dehydrogenase and a platinum electrode take place via the conducting-polymer network, mechanism modelling, overview | Gluconobacter sp. DSM 3504 / ATCC 15163 | acetaldehyde + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Gluconobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
QH-ADH | - |
Gluconobacter sp. |
quinohemoprotein alcohol dehydrogenase | - |
Gluconobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Gluconobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Gluconobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | eight molecules per enzyme molecule | Gluconobacter sp. | |
pyrroloquinoline quinone | PQQ, two molecules per enzyme molecule | Gluconobacter sp. |