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Literature summary for 1.1.5.7 extracted from

  • Moonmangmee, D.; Fujii, Y.; Toyama, H.; Theeragool, G.; Lotong, N.; Matsushita, K.; Adachi, O.
    Purification and characterization of membrane-bound quinoprotein cyclic alcohol dehydrogenase from Gluconobacter frateurii CHM 9 (2001), Biosci. Biotechnol. Biochem., 65, 2763-2772.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
Cyclopentanol pH 5.5, 25°C Gluconobacter frateurii

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm Gluconobacter frateurii 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ addition of pyrroloquinoline quinone and Ca2+ converts the apo-enzyme to the holoenzyme Gluconobacter frateurii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
x * 83000, SDS-PAGE Gluconobacter frateurii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gluconobacter frateurii the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible ?
-
?
additional information Gluconobacter frateurii CHM 9 the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible ?
-
?

Organism

Organism UniProt Comment Textmining
Gluconobacter frateurii
-
CHM 9
-
Gluconobacter frateurii CHM 9
-
CHM 9
-

Purification (Commentary)

Purification (Comment) Organism
-
Gluconobacter frateurii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
44.3
-
-
Gluconobacter frateurii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R,2R)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone 74% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
(1R,2R)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone 74% of the activity compared to cyclopentanol Gluconobacter frateurii CHM 9 ?
-
ir
(1S,2S)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone 11% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
(1S,2S)-trans-1,2-cyclohexanediol + pyrroloquinoline quinone 11% of the activity compared to cyclopentanol Gluconobacter frateurii CHM 9 ?
-
ir
(2R,3R)-2,3-butanediol + pyrroloquinoline quinone 41% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1,2-butanediol + pyrroloquinoline quinone 63% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1,3-butanediol + pyrroloquinoline quinone 12% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1,3-cyclopentanediol + pyrroloquinoline quinone 73% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
1,4-cyclohexanediol + pyrroloquinoline quinone 14% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
2,3-butanediol + pyrroloquinoline quinone 186% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
2,4-pentanediol + pyrroloquinoline quinone 16% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
2-butanol + pyrroloquinoline quinone 41% of the activity compared to cyclopentanol Gluconobacter frateurii butane-2-one + reduced pyrroloquinoline quinone
-
ir
2-butanol + pyrroloquinoline quinone 41% of the activity compared to cyclopentanol Gluconobacter frateurii CHM 9 butane-2-one + reduced pyrroloquinoline quinone
-
ir
2-hexanol + pyrroloquinoline quinone 10% of the activity compared to cyclopentanol Gluconobacter frateurii 2-hexanone + pyrroloquinoline quinol
-
ir
2-methyl-2,4-pentanediol + pyrroloquinoline quinone 17% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
2-propanol + pyrroloquinoline quinone 17% of the activity compared to cyclopentanol Gluconobacter frateurii acetone + pyrroloquinoline quinol
-
ir
3-pentanol + pyrroloquinoline quinone 74% of the activity compared to cyclopentanol Gluconobacter frateurii 3-pentanone + pyrroloquinoline quinol
-
ir
cis-1,2-cyclohexanediol + pyrroloquinoline quinone 88% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
cis-1,2-cyclopentanediol + pyrroloquinoline quinone 181% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
cis-4-cyclopentene-1,3-diol + pyrroloquinoline quinone 32% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
cyclobutanol + pyrroloquinoline quinone 73% of the activity compared to cyclopentanol Gluconobacter frateurii cyclobutanone + pyrroloquinoline quinol
-
ir
cyclohexanol + pyrroloquinoline quinone 73% of the activity compared to cyclopentanol Gluconobacter frateurii cyclohexanone + pyrroloquinoline quinol
-
ir
cyclooctanol + pyrroloquinoline quinone 137% of the activity compared to cyclopentanol Gluconobacter frateurii cyclooctanone + pyrroloquinoline quinol
-
ir
cyclopentanol + pyrroloquinoline quinone only pyrroloquinoline quinone is effective as electron acceptor, no activity with FAD, FMN and NAD(P)+ Gluconobacter frateurii cyclopentanone + pyrroloquinoline quinol
-
ir
D-arabitol + pyrroloquinoline quinone 78% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
D-mannitol + pyrroloquinoline quinone 25% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
D-sorbitol + pyrroloquinoline quinone 34% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
glycerol + pyrroloquinoline quinone 59% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
meso-erythritol + pyrroloquinoline quinone 100% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir
additional information the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible Gluconobacter frateurii ?
-
?
additional information the enzyme is unable to catalyze the reverse reaction of cyclic ketones or aldehydes to cyclic alcohols. This enzyme oxidizes a wide variety of cyclic alcohols. Some minor enzyme activity is found with aliphatic secondary alcohols and sugar alcohols, but not primary alcohols Gluconobacter frateurii ?
-
?
additional information the localization of the enzyme on the outer surface of the organism is advantageous to facilitate the oxidative fermentation of the cyclic alcohols. Since cyclic alcohols have some biological toxicity to living cells, according to the mechanism of the oxidative fermentation, there is no need to incorporate such toxic compounds into the cytoplasm to oxidize and pump out the oxidation products across the cytoplasmic membrane by the expense of bioenergy. The enzyme not inducible Gluconobacter frateurii CHM 9 ?
-
?
additional information the enzyme is unable to catalyze the reverse reaction of cyclic ketones or aldehydes to cyclic alcohols. This enzyme oxidizes a wide variety of cyclic alcohols. Some minor enzyme activity is found with aliphatic secondary alcohols and sugar alcohols, but not primary alcohols Gluconobacter frateurii CHM 9 ?
-
?
ribitol + pyrroloquinoline quinone 34% of the activity compared to cyclopentanol Gluconobacter frateurii ?
-
ir

Subunits

Subunits Comment Organism
? x * 83000, SDS-PAGE Gluconobacter frateurii

Synonyms

Synonyms Comment Organism
MCAD
-
Gluconobacter frateurii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Gluconobacter frateurii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Gluconobacter frateurii

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone only pyrroloquinoline quinone is effective as electron acceptor, no activity with FAD, FMN and NAD(P)+ Gluconobacter frateurii