Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-benzoquinone | competitive inhibition of the activity with 2,6-dichlorophenolindophenol or coenzyme Q1, non-competitive inhibition of reaction with phenazine methosulfate | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
coenzyme Q1 | - |
Pseudomonas sp. | |
0.13 | - |
phenazine methosulfate | - |
Pseudomonas sp. | |
0.47 | - |
D-glucose | reaction with 2,6-dichlorophenolindophenol at pH 6.0 | Pseudomonas sp. | |
0.56 | - |
tetramethyl-p-phenylenediamine | - |
Pseudomonas sp. | |
0.69 | - |
ferricyanide | - |
Pseudomonas sp. | |
1.6 | - |
2,6-dichlorophenolindophenol | - |
Pseudomonas sp. | |
6.3 | - |
D-glucose | reaction with phenazine methosulfate/2,6-dichlorophenolindophenol at pH 8.75 | Pseudomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic membrane | bound to | Pseudomonas sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
1 * 87000, monomeric in presence of 1% Triton X-100, aggregation after removing the detergent, urea-SDS-PAGE | Pseudomonas sp. |
93000 | - |
sucrose density gradient centrifugation, in presence of Triton X-100 | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + acceptor | 6.5% of the activity with D-glucose | Pseudomonas sp. | D-galactono-1,5-lactone + reduced acceptor | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | - |
Pseudomonas sp. | D-gluconic acid + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + coenzyme Q1 | - |
Pseudomonas sp. | D-glucono-1,5-lactone + reduced coenzyme Q1 | - |
? | |
D-glucose + ferricyanide | - |
Pseudomonas sp. | D-glucono-1,5-lactone + ferrocyanide | - |
? | |
D-glucose + phenazine methosulfate | - |
Pseudomonas sp. | D-glucono-1,5-lactone + reduced phenazine methosulfate | - |
? | |
D-glucose + tetramethyl-p-phenylenediamine | - |
Pseudomonas sp. | D-glucono-1,5-lactone + reduced tetramethyl-p-phenylenediamine | - |
? | |
D-mannose + acceptor | 8.6% of the activity with D-glucose | Pseudomonas sp. | D-manno-1,5-lactone + reduced acceptor | - |
? | |
D-xylose + acceptor | 13% of the activity with D-glucose | Pseudomonas sp. | D-xylono-1,5-lactone + reduced acceptor | - |
? | |
L-arabinose + acceptor | 2.8% of the activity with D-glucose | Pseudomonas sp. | L-arabinono-1,5-lactone + reduced acceptor | - |
? | |
L-rhamnose + acceptor | 7.5% of the activity with D-glucose | Pseudomonas sp. | L-rhamnone-1,5-lactone + reduced acceptor | - |
? | |
maltose + acceptor | 3.2% of the activity with D-glucose | Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 87000, monomeric in presence of 1% Triton X-100, aggregation after removing the detergent, urea-SDS-PAGE | Pseudomonas sp. |
More | aggregation in medium containing no Triton X-100 to dimers, trimers and tetramers | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
reaction with ferricyanide | Pseudomonas sp. |
6 | - |
reaction with 2,6-dichlorophenolindophenol | Pseudomonas sp. |
6.5 | - |
reaction with coenzyme Q1 | Pseudomonas sp. |
8.8 | - |
reaction with phenazine methosulfate or tetramethyl-p-phenylenediamine | Pseudomonas sp. |