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Literature summary for 1.1.5.9 extracted from

  • Yamaoka, H.; Ferri, S.; Fujikawa, M.; Sode, K.
    Essential role of the small subunit of thermostable glucose dehydrogenase from Burkholderia cepacia (2004), Biotechnol. Lett., 26, 1757-1761.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as recombinant protein in Escherichia coli strain DH5alpha Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
SDS-PAGE, X * 60000 (alpha-subunit), x * 40000 (beta-subunit), x * 18000 (gamma-subunit), x * 13000 (gamma-subunit) Burkholderia cepacia
18000
-
SDS-PAGE, X * 60000 (alpha-subunit), x * 40000 (beta-subunit), x * 18000 (gamma-subunit), x * 13000 (gamma-subunit) Burkholderia cepacia
40000
-
SDS-PAGE, X * 60000 (alpha-subunit), x * 40000 (beta-subunit), x * 18000 (gamma-subunit), x * 13000 (gamma-subunit) Burkholderia cepacia
60000
-
SDS-PAGE, X * 60000 (alpha-subunit), x * 40000 (beta-subunit), x * 18000 (gamma-subunit), x * 13000 (gamma-subunit) Burkholderia cepacia

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
-
-
Burkholderia cepacia SM4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + 2,6-dichlorophenolindophenol
-
Burkholderia cepacia D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + 2,6-dichlorophenolindophenol
-
Burkholderia cepacia SM4 D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + phenazine methosulfate
-
Burkholderia cepacia D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?
D-glucose + phenazine methosulfate
-
Burkholderia cepacia SM4 D-glucono-1,5-lactone + reduced phenazine methosulfate
-
?

Subunits

Subunits Comment Organism
heteromer SDS-PAGE, X * 60000 (alpha-subunit), x * 40000 (beta-subunit), x * 18000 (gamma-subunit), x * 13000 (gamma-subunit) Burkholderia cepacia

Synonyms

Synonyms Comment Organism
GDH
-
Burkholderia cepacia
thermostable glucose dehydrogenase
-
Burkholderia cepacia