Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Burkholderia cepacia | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
after preincubation at temperatures below 45°C: x * 67000 (alpha-subunit) x * 43000 (beta subunit), SDS-PAGE | Burkholderia cepacia |
67000 | - |
after preincubation at 70°C for 30 min: x * 67000 (alpha-subunit), SDS-PAGE | Burkholderia cepacia |
67000 | - |
after preincubation at temperatures below 45°C: x * 67000 (alpha-subunit) x * 43000 (beta subunit), SDS-PAGE | Burkholderia cepacia |
85000 | - |
after preincubation at 70°C for 30 min, native PAGE | Burkholderia cepacia |
350000 | - |
native PAGE | Burkholderia cepacia |
380000 | - |
gel filtration chromatography | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | - |
bacterial strain SM4 is used, isolated from a soil sample near a Japanese hot spring | - |
Purification (Comment) | Organism |
---|---|
- |
Burkholderia cepacia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6 | - |
at 75°C after preincubation of the enzyme at 70°C for 30 min | Burkholderia cepacia |
15 | - |
at 70°C | Burkholderia cepacia |
45 | - |
at 45°C | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + 2,6-dichlorophenolindophenol | - |
Burkholderia cepacia | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
heteromer | after preincubation at temperatures below 45°C: x * 67000 (alpha-subunit) x * 43000 (beta subunit), SDS-PAGE | Burkholderia cepacia |
homooligomer | after preincubation at 70°C for 30 min: x * 67000 (alpha-subunit), SDS-PAGE | Burkholderia cepacia |
Synonyms | Comment | Organism |
---|---|---|
thermostable GDH | - |
Burkholderia cepacia |
thermostable glucose dehydrogenase | - |
Burkholderia cepacia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
enzyme activity shows a second peak at 70°C with 30% of maximal activity | Burkholderia cepacia |
75 | - |
after preincubating the enzyme at 70°C for 30 min | Burkholderia cepacia |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 85 | 20% of maximal activity at 20°C and 10% of maximal activity at 85°C, maximal activity is at 45°C with a second activity peak at 70°C (30% of maximal activity) | Burkholderia cepacia |
40 | 85 | after preincubation of the enzyme at 70°C for 30 min: 15% of maximal activity at 40°C and 50% of maximal activity at 85°C, maximal activity at 75°C | Burkholderia cepacia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 70 | enzyme is stable at temperatures between 5-37°C for 30 min, after incubating enzyme at 45°C for 30 min 40% of enzyme activity remains, at 70°C for 30 min 10% of enzyme activity remains | Burkholderia cepacia |
5 | 75 | after preincubation at 70°C for 30 min the enzyme is stable at temperatures between 5-50°C for 30 min, after incubating enzyme at 70°C for 30 min 70% of activity remains | Burkholderia cepacia |