Any feedback?
Literature summary for 1.1.5.9 extracted from
- Reduction of quinones and phenoxy radicals by extracellular glucose dehydrogenase from Glomerella cingulata suggests a role in plant pathogenicity (2011), Microbiology, 157, 3203-3212.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, phylogenetic analysis | Colletotrichum gloeosporioides |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Colletotrichum gloeosporioides | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
1 * 68000, deglycosylated enzyme, SDS-PAGE | Colletotrichum gloeosporioides |
| 95000 | 135000 | glycoprotein, native PAGE | Colletotrichum gloeosporioides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + a quinone | Colletotrichum gloeosporioides | - |
D-glucono-1,5-lactone + a quinol | - |
? |  |
| D-glucose + a quinone | Colletotrichum gloeosporioides DSM 62728 | - |
D-glucono-1,5-lactone + a quinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Colletotrichum gloeosporioides | - |
anamorph Colletotrichum gloeosporoides | - |
| Colletotrichum gloeosporioides DSM 62728 | - |
anamorph Colletotrichum gloeosporoides | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | four potential N-glycosylation sites at N55, N233, N255 and N339 | Colletotrichum gloeosporioides |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 23.7fold from culture supernatant by anion exchange and hydrophobic interaction chromatography, ammonium sulfate fractionation, and ultrafiltration | Colletotrichum gloeosporioides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 565 | - |
purified enzyme with ferrocenium hexafluorophosphate, pH 5.5, 30°C | Colletotrichum gloeosporioides |
| 840 | - |
purified enzyme with 2,6-dichlorophenol indophenol , pH 5.5, 30°C | Colletotrichum gloeosporioides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + 1,4-benzoquinone | - |
Colletotrichum gloeosporioides | D-glucono-1,5-lactone + 1,4-benzoquinol | - |
? | |
| D-glucose + 1,4-benzoquinone | - |
Colletotrichum gloeosporioides DSM 62728 | D-glucono-1,5-lactone + 1,4-benzoquinol | - |
? | |
| D-glucose + a quinone | - |
Colletotrichum gloeosporioides | D-glucono-1,5-lactone + a quinol | - |
? | |
| D-glucose + a quinone | - |
Colletotrichum gloeosporioides DSM 62728 | D-glucono-1,5-lactone + a quinol | - |
? | |
| D-glucose + ferricenium hexafluorophosphate | - |
Colletotrichum gloeosporioides | D-glucono-1,5-lactone + ferrocenium hexafluorophosphate | - |
? | |
| D-glucose + ferricenium hexafluorophosphate | - |
Colletotrichum gloeosporioides DSM 62728 | D-glucono-1,5-lactone + ferrocenium hexafluorophosphate | - |
? | |
| D-glucose + ferricyanide | - |
Colletotrichum gloeosporioides | D-glucono-1,5-lactone + ferrocyanide | - |
? | |
| D-glucose + ferricyanide | - |
Colletotrichum gloeosporioides DSM 62728 | D-glucono-1,5-lactone + ferrocyanide | - |
? | |
| D-glucose + oxidized 2,6-dichlorophenol indophenol | - |
Colletotrichum gloeosporioides | D-glucono-1,5-lactone + reduced 2,6-dichlorophenol indophenol | - |
? | |
| additional information | suitable electron acceptors are quinones, phenoxy radicals, 2,6-dichloroindophenol, ferricyanide and ferrocenium hexafluorophosphate. Reduction of quinones and phenoxy radicals by extracellular enzyme, overview | Colletotrichum gloeosporioides | ? | - |
? | |
| additional information | suitable electron acceptors are quinones, phenoxy radicals, 2,6-dichloroindophenol, ferricyanide and ferrocenium hexafluorophosphate. Reduction of quinones and phenoxy radicals by extracellular enzyme, overview | Colletotrichum gloeosporioides DSM 62728 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 68000, deglycosylated enzyme, SDS-PAGE | Colletotrichum gloeosporioides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAD-dependent glucose dehydrogenase | - |
Colletotrichum gloeosporioides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Colletotrichum gloeosporioides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at with 2,6-dichlorophenol indophenol or ferrocenium hexafluorophosphate | Colletotrichum gloeosporioides |
| 7.5 | - |
assay at with 1,4-benzoquinone | Colletotrichum gloeosporioides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | dependent on, non-covalently bound | Colletotrichum gloeosporioides | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Colletotrichum gloeosporioides | isoelectric focusing | - |
5.6 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the to the GMC family of oxidoreductases, phylogenetic analysis | Colletotrichum gloeosporioides |
| physiological function | the enzyme from Glomerella cingulata might play a role in plant pathogenicity. The quinone and phenoxy radical reducing enzyme light act to neutralize the action of plant laccase, phenoloxidase or peroxidase activities, which are increased in infected plants to evade fungal attack | Colletotrichum gloeosporioides |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
