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Literature summary for 1.1.98.4 extracted from

  • Abken, H.-J.; Deppenmeier, U.
    Purification and properties of an F420H2 dehydrogenase from Methanosarcina mazei Gö1 (1997), FEMS Microbiol. Lett., 154, 231-237.
No PubMed abstract available
Search for more literature for 1.1.98.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 reduced coenzyme F420 pH 8.5, 39°C Methanosarcina mazei Gö1

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Methanosarcina mazei Gö1 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron-sulfur cluster the enzyme contains 7 mol S2- and 7 mol Fe Methanosarcina mazei Gö1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 x * 40000 + x * 37000 + x * 22000 + x * 20000 x * 16000, SDS-PAGE Methanosarcina mazei Gö1
20000
-
 x * 40000 + x * 37000 + x * 22000 + x * 20000 x * 16000, SDS-PAGE Methanosarcina mazei Gö1
22000
-
 x * 40000 + x * 37000 + x * 22000 + x * 20000 x * 16000, SDS-PAGE Methanosarcina mazei Gö1
37000
-
 x * 40000 + x * 37000 + x * 22000 + x * 20000 x * 16000, SDS-PAGE Methanosarcina mazei Gö1
40000
-
 x * 40000 + x * 37000 + x * 22000 + x * 20000 x * 16000, SDS-PAGE Methanosarcina mazei Gö1
115000
-
 gel filtration Methanosarcina mazei Gö1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Methanosarcina mazei Gö1 the enzyme is involved in the reduction of heterodisulfide ?
-
 ?
additional information Methanosarcina mazei Gö1 DSM 3647 the enzyme is involved in the reduction of heterodisulfide ?
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei Gö1
-
-
-
Methanosarcina mazei Gö1 DSM 3647
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina mazei Gö1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17
-
 activity with reduced coenzyme F420 as electron donor and methylviologen and metronidazole as electron acceptors at pH 8.5 at a temperature of 39°C Methanosarcina mazei Gö1
68
-
 activity with reduced coenzyme F420 as electron donor and tetramethyl-4-benzoquinone as electron acceptors at pH 8.5 at a temperature of 39°C Methanosarcina mazei Gö1
136
-
 activity with reduced coenzyme F420 as electron donor and 2,3-dimethyl-1,4-naphthoquinone as electron acceptors at pH 8.5 at a temperature of 39°C Methanosarcina mazei Gö1
173
-
 activity with reduced coenzyme F420 as electron donor and 2-methyl-1,4-naphthoquinone as electron acceptors at pH 8.5 at a temperature of 39°C Methanosarcina mazei Gö1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dimethyl-1,4-naphthoquinone + reduced coenzyme F420
-
 Methanosarcina mazei Gö1 2,3-dimethyl-1,4-naphthoquinol + oxidized coenzyme F420
-
 ?
2,3-dimethyl-1,4-naphthoquinone + reduced coenzyme F420
-
 Methanosarcina mazei Gö1 DSM 3647 2,3-dimethyl-1,4-naphthoquinol + oxidized coenzyme F420
-
 ?
2-methyl-1,4-naphthoquinone + reduced coenzyme F420 i.e. menadione Methanosarcina mazei Gö1 2-methyl-1,4-naphthoquinol + oxidized coenzyme F420
-
 ?
2-methyl-1,4-naphthoquinone + reduced coenzyme F420 i.e. menadione Methanosarcina mazei Gö1 DSM 3647 2-methyl-1,4-naphthoquinol + oxidized coenzyme F420
-
 ?
additional information the enzyme is involved in the reduction of heterodisulfide Methanosarcina mazei Gö1 ?
-
 ?
additional information no hydrogenase activity. No activity with NAD+ or NADP+ Methanosarcina mazei Gö1 ?
-
 ?
additional information the enzyme is involved in the reduction of heterodisulfide Methanosarcina mazei Gö1 DSM 3647 ?
-
 ?
additional information no hydrogenase activity. No activity with NAD+ or NADP+ Methanosarcina mazei Gö1 DSM 3647 ?
-
 ?
tetramethyl-4-benzoquinone + reduced coenzyme F420 i.e. menadione Methanosarcina mazei Gö1 tetramethyl-1,4-benzoquinol + oxidized coenzyme F420
-
 ?
tetramethyl-4-benzoquinone + reduced coenzyme F420 i.e. menadione Methanosarcina mazei Gö1 DSM 3647 tetramethyl-1,4-benzoquinol + oxidized coenzyme F420
-
 ?

Subunits

Subunits Comment Organism
oligomer x * 40000 + x * 37000 + x * 22000 + x * 20000 x * 16000, SDS-PAGE Methanosarcina mazei Gö1

Synonyms

Synonyms Comment Organism
F420H2 dehydrogenase part of the energy-conserving F420H2:heterodisulfide oxidoreductase system (EC 1.8.98.1) Methanosarcina mazei Gö1

Cofactor

Cofactor Comment Organism Structure
FAD contains 0.2 mol FAD per mol of enzyme Methanosarcina mazei Gö1

General Information

General Information Comment Organism
physiological function the enzyme is involved in the reduction of heterodisulfide Methanosarcina mazei Gö1