Any feedback?
Literature summary for 1.1.99.12 extracted from
- Enzymatic studies on the oxidation of sugar and sugar alcohol. 8. Particle-bound L-sorbose dehydrogenase from Gluconobacter suboxydans (1969), J. Biochem., 66, 521-527.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| azide | 1 mM, 30-40% increase in activity, 2,6-dichlorophenol as electron acceptor | Gluconobacter oxydans |  |
| cyanide | 1 mM, 30-40% increase in activity, 2,6-dichlorophenol as electron acceptor | Gluconobacter oxydans | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, complete inhibition | Gluconobacter oxydans | |
| Cu2+ | 1 mM, complete inhibition | Gluconobacter oxydans | |
| Hg2+ | 1 mM, complete inhibition | Gluconobacter oxydans | |
| p-chloromercuribenzoate | 1 mM, 40% inhibition | Gluconobacter oxydans | |
| Phenylmercuric nitrate | 1 mM, 40% inhibition | Gluconobacter oxydans | |
| Semicarbazide | 1 mM, 40% inhibition | Gluconobacter oxydans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.3 | - |
L-sorbose | - |
Gluconobacter oxydans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| particle-bound | - |
Gluconobacter oxydans | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-sorbose + 2,6-dichlorophenolindophenol | Gluconobacter oxydans | no activity with D-fructose, sucrose and dulcitol | 5-keto-D-fructose + reduced 2,6-dichlorophenolindophenol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0099 | - |
- |
Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose + 2,6-dichlorophenolindophenol | 180% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| D-gluconate + 2,6-dichlorophenolindophenol | 135% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| D-glucose + 2,6-dichlorophenolindophenol | 382% of activity with L-sorbose | Gluconobacter oxydans | 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-mannitol + 2,6-dichlorophenolindophenol | 328% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| D-mannose + 2,6-dichlorophenolindophenol | 190% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| D-sorbitol + 2,6-dichlorophenolindophenol | 728% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| D-xylose + 2,6-dichlorophenolindophenol | 355% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| glycerol + 2,6-dichlorophenolindophenol | 327% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| iso-erythritol + 2,6-dichlorophenolindophenol | 236% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| L-arabinose + 2,6-dichlorophenolindophenol | 318% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| L-sorbose + 2,6-dichlorophenolindophenol | no activity with D-fructose, sucrose and dulcitol | Gluconobacter oxydans | 5-keto-D-fructose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| maltose + 2,6-dichlorophenolindophenol | 282% of activity with L-sorbose | Gluconobacter oxydans | 4-O-alpha-D-ribo-hexopyranosyl-3-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Gluconobacter oxydans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
5 min, complete inactivation | Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
- |
Gluconobacter oxydans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.7 | pH 5.0: about 40% of activity maximum, pH 8.7: about 30% of activity maximum | Gluconobacter oxydans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
30 min, 20°C, complete inactivation | Gluconobacter oxydans |
| 6 | 6.5 | 30 min, 20°C, highest stability | Gluconobacter oxydans |
| 7.5 | - |
30 min, 20°C, complete inactivation | Gluconobacter oxydans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
