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Literature summary for 1.1.99.14 extracted from

  • Isobe, K.; Watabe, S.; Yamada, M.
    Characterization and application of a glycolate dehydrogenase from Trichoderma harzianum AIU 353 (2012), J. Mol. Catal. B, 83, 94-99.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
8-oxyquinoline 80% residual activity at 1 mM Trichoderma harzianum
Co2+ 88% residual activity at 1 mM Trichoderma harzianum
Cu2+ complete inhibition at 1 mM Trichoderma harzianum
DW 1750000 91% residual activity at 1 mM Trichoderma harzianum
Fe3+ 73% residual activity at 1 mM Trichoderma harzianum
glyoxylate 79% residual activity at 1 mM Trichoderma harzianum
Hg2+ 2% residual activity at 1 mM Trichoderma harzianum
hydrazine 77% residual activity at 1 mM Trichoderma harzianum
hydroxylamine 83% residual activity at 1 mM Trichoderma harzianum
iodoacetic acid 63% residual activity at 1 mM Trichoderma harzianum
Mn2+ complete inhibition at 1 mM Trichoderma harzianum
additional information not inhibited by semicarbazide, 4-chloromercuribenzoic acid and EDTA Trichoderma harzianum
N-ethylmaleimide 56% residual activity at 1 mM Trichoderma harzianum
Ni2+ complete inhibition at 1 mM Trichoderma harzianum
o-phenanthroline 88% residual activity at 1 mM Trichoderma harzianum
Sodium azide 85% residual activity at 1 mM Trichoderma harzianum
Zn2+ 76% residual activity at 1 mM Trichoderma harzianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
Nitro blue tetrazolium at pH 7.0 and 30°C Trichoderma harzianum
0.94
-
glycolate at pH 7.0 and 30°C Trichoderma harzianum
3.6
-
L-lactate at pH 7.0 and 30°C Trichoderma harzianum
49.5
-
dihydroxyacetone at pH 7.0 and 30°C Trichoderma harzianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE Trichoderma harzianum
53000
-
1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE Trichoderma harzianum
81000
-
1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE Trichoderma harzianum
107000
-
1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE Trichoderma harzianum
280000
-
gel filtration Trichoderma harzianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trichoderma harzianum the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert ?
-
?
additional information Trichoderma harzianum AIU 353 the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert ?
-
?

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
-
-
Trichoderma harzianum AIU 353
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Toyopearl column chromatography, Q-Toyopearl column chromatography, and Toyopearl, GigaCap Q-Toyopearl column chromatography, hydroxyapatite column chromatography, and gel filtration Trichoderma harzianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00165
-
crude cell extract, at pH 7.0 and 30°C Trichoderma harzianum
0.727
-
after 440fold purification, at pH 7.0 and 30°C Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone + nitro blue tetrazolium 162% activity compared to glycolate Trichoderma harzianum ? + reduced nitro blue tetrazolium
-
?
dihydroxyacetone + nitro blue tetrazolium 162% activity compared to glycolate Trichoderma harzianum AIU 353 ? + reduced nitro blue tetrazolium
-
?
DL-glyceraldehyde + nitro blue tetrazolium 78% activity compared to glycolate Trichoderma harzianum ? + reduced nitro blue tetrazolium
-
?
DL-glyceraldehyde + nitro blue tetrazolium 78% activity compared to glycolate Trichoderma harzianum AIU 353 ? + reduced nitro blue tetrazolium
-
?
DL-lactate + nitro blue tetrazolium 235% activity compared to glycolate Trichoderma harzianum ? + reduced nitro blue tetrazolium
-
?
DL-lactate + nitro blue tetrazolium 235% activity compared to glycolate Trichoderma harzianum AIU 353 ? + reduced nitro blue tetrazolium
-
?
glycolaldehyde + nitro blue tetrazolium 38% activity compared to glycolate Trichoderma harzianum ? + reduced nitro blue tetrazolium
-
?
glycolate + 2,6-dichlorophenolindophenol
-
Trichoderma harzianum glyoxylate + reduced 2,6-dichlorophenolindophenol
-
?
glycolate + 2,6-dichlorophenolindophenol
-
Trichoderma harzianum AIU 353 glyoxylate + reduced 2,6-dichlorophenolindophenol
-
?
glycolate + nitro blue tetrazolium 100% activity Trichoderma harzianum glyoxylate + reduced nitro blue tetrazolium
-
?
L-lactate + nitro blue tetrazolium 232% activity compared to glycolate Trichoderma harzianum ? + reduced nitro blue tetrazolium
-
?
methyl glycolate + nitro blue tetrazolium 82% activity compared to glycolate Trichoderma harzianum methyl glyoxylate + reduced nitro blue tetrazolium
-
?
additional information the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert Trichoderma harzianum ?
-
?
additional information the enzyme does not require NAD+ and NADP+ as an electron carrier, and primary alcohols, dihydric alcohols and aliphatic aldehydes are not oxidized. Ethanol, 1-propanol, 2-propanol, 1-butanol, ethylene glycol, propylene glycol, 1,3-propanediol, glycerol, glyoxylate, glyoxal, methylglyoxal, acetaldehyde, propylaldehyde, L-malic acid, L-kactic acid, D-malic acid and L-tartaric acid are inert Trichoderma harzianum AIU 353 ?
-
?

Subunits

Subunits Comment Organism
heterotetramer 1 * 107000 + 1 * 81000 + 1 * 53000 + 1 * 38000, SDS-PAGE Trichoderma harzianum

Synonyms

Synonyms Comment Organism
GADH
-
Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Trichoderma harzianum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 when the enzyme is incubated with 0.1 M potassium phosphate buffer, pH 6.0, at 20–60°C for 30 min, approximately 90% of the enzyme activity remain below 20°C, but the activity completely disappears at 40°C Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Trichoderma harzianum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6.5 more than 80% of enzyme activity remains in the pH range between 5.0 and 6.5 for 20 min at 20°C Trichoderma harzianum

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma harzianum isoelectric focusing
-
5.1