Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermothelomyces heterothallicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00067 | - |
cytochrome c | at 25°C | Thermothelomyces heterothallicus | |
0.00067 | - |
cytochrome c | at pH 4.5 and 25°C | Thermothelomyces heterothallicus | |
0.001 | - |
cytochrome c | at 45°C | Thermothelomyces heterothallicus | |
0.001 | - |
cytochrome c | at pH 4.5 and 45°C | Thermothelomyces heterothallicus | |
0.0024 | - |
2,6-dichlorophenolindophenol | at 25°C | Thermothelomyces heterothallicus | |
0.0024 | - |
2,6-dichlorophenolindophenol | at pH 4.5 and 25°C | Thermothelomyces heterothallicus | |
0.0034 | - |
cellobiose | at 25°C | Thermothelomyces heterothallicus | |
0.0037 | - |
2,6-dichlorophenolindophenol | at 45°C | Thermothelomyces heterothallicus | |
0.0037 | - |
2,6-dichlorophenolindophenol | at pH 4.5 and 45°C | Thermothelomyces heterothallicus | |
0.0045 | - |
ferricyanide | at 25°C | Thermothelomyces heterothallicus | |
0.0045 | - |
ferricyanide | at pH 4.5 and 25°C | Thermothelomyces heterothallicus | |
0.0058 | - |
cellobiose | at 25°C | Thermothelomyces heterothallicus | |
0.0064 | - |
cellotetraose | at 25°C | Thermothelomyces heterothallicus | |
0.0067 | - |
ferricyanide | at 45°C | Thermothelomyces heterothallicus | |
0.0067 | - |
ferricyanide | at pH 4.5 and 45°C | Thermothelomyces heterothallicus | |
0.011 | - |
cellotetraose | at 25°C | Thermothelomyces heterothallicus | |
0.0281 | - |
lactose | at 25°C | Thermothelomyces heterothallicus | |
0.05 | - |
lactose | at 45°C | Thermothelomyces heterothallicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
extracellular | - |
Thermothelomyces heterothallicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
gel filtration | Thermothelomyces heterothallicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Thermothelomyces heterothallicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Phanerodontia chrysosporium |
glycoprotein | difference in apparent molecular weight and that observed by SDS-PAGE might be due to glycosylation, six potential glycosylation sites in enzyme amino acid sequence | Thermothelomyces heterothallicus |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Phenyl-Sepharose, Sephacryl S-200, Mono Q | Thermothelomyces heterothallicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Phanerodontia chrysosporium | - |
culture medium | - |
Thermothelomyces heterothallicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.3 | - |
at pH 4.0 and 25°C | Thermothelomyces heterothallicus |
11.5 | - |
at 40°C | Phanerodontia chrysosporium |
13.4 | - |
cytochrome c reduction at 60°C | Thermothelomyces heterothallicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichlorophenolindophenol | - |
Thermothelomyces heterothallicus | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
r | |
cellobiose + acceptor | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
cellobiose + acceptor | electron acceptors: phenol blue, Fe3+, methylene blue, 3,5-di-tert-butyl-1,2-benzoquinone, p-benzoquinone, potassium ferricyanide, cytochrome c | Thermothelomyces heterothallicus | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
cellobiose + cytochrome c | - |
Thermothelomyces heterothallicus | cellobiono-1,5-lactone + reduced cytochrome c | - |
r | |
cellobiose + ferricyanide | - |
Thermothelomyces heterothallicus | cellobiono-1,5-lactone + ferrocyanide | - |
r | |
cellotetraose + acceptor | - |
Phanerodontia chrysosporium | cellotetrono-1,5-lactone + reduced acceptor | - |
? | |
cellotetraose + acceptor | - |
Thermothelomyces heterothallicus | cellotetrono-1,5-lactone + reduced acceptor | - |
? | |
lactose + acceptor | - |
Phanerodontia chrysosporium | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
lactose + acceptor | - |
Thermothelomyces heterothallicus | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 95000, SDS-PAGE | Thermothelomyces heterothallicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Phanerodontia chrysosporium |
60 | - |
- |
Thermothelomyces heterothallicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | thermostable enzyme, 10 h half-life at 60°C, 3 h at 70°C | Thermothelomyces heterothallicus |
60 | - |
25 min half-life at 60°C, instantaneousley inactivated at 70°C | Phanerodontia chrysosporium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
lactose | at 25°C | Thermothelomyces heterothallicus | |
6.1 | - |
cellobiose | at 25°C | Thermothelomyces heterothallicus | |
6.1 | - |
cellotetraose | at 25°C | Thermothelomyces heterothallicus | |
7.3 | - |
cytochrome c | at 25°C | Thermothelomyces heterothallicus | |
7.5 | - |
2,6-dichlorophenolindophenol | at 25°C | Thermothelomyces heterothallicus | |
12.8 | - |
ferricyanide | at 25°C | Thermothelomyces heterothallicus | |
15.1 | - |
cellotetraose | at 25°C | Thermothelomyces heterothallicus | |
16.1 | - |
cellobiose | at 25°C | Thermothelomyces heterothallicus | |
16.8 | - |
lactose | at 45°C | Thermothelomyces heterothallicus | |
18.3 | - |
2,6-dichlorophenolindophenol | at 45°C | Thermothelomyces heterothallicus | |
20.8 | - |
cytochrome c | at 45°C | Thermothelomyces heterothallicus | |
24.1 | - |
ferricyanide | at 45°C | Thermothelomyces heterothallicus |