Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.99.18 extracted from

  • Ludwig, R.; Haltrich, D.
    Optimisation of cellobiose dehydrogenase production by the fungus Sclerotium (Athelia) rolfsii (2003), Appl. Microbiol. Biotechnol., 61, 32-39.
    View publication on PubMed
Search for more literature for 1.1.99.18

Application

Application Comment Organism
synthesis the enzyme contains a protease-sensitive linker region, can be cleaved by endogenous proteases into a catalytically active flavin fragment and an inactive haem domain. Cleavage can be prevented by using increased concentrations of peptone or certain amino acids such as Val or Leu. These concentrations of up to 80 g peptone per l are not realistic for large scale-fermentations. Yet high levels of the enzyme produced under these conditions should stimulate both basic studies on the enzyme and studies on potential technological applications, e.g. for biosensors, bioremediation, or biocatalysis Athelia rolfsii

General Stability

General Stability Organism
the enzyme contains a protease-sensitive linker region, can be cleaved by endogenous proteases into a catalytically active flavin fragement and an inactive haem domain. Cleavage can be prevented by using increased concentrations of peptone or certain amino acids such as Val or Leu Athelia rolfsii

Organism

Organism UniProt Comment Textmining
Athelia rolfsii
-
 Athelia, CBS 191.62, very efficient producer of the enzyme
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + 2,6-dichlorophenol-indophenol
-
 Athelia rolfsii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol
-
 ?

Synonyms

Synonyms Comment Organism
Cdh
-
 Athelia rolfsii

Cofactor

Cofactor Comment Organism Structure
flavin the enzyme contains a protease-sensitive linker region, can be cleaved by endogenous proteases into a catalytically active flavin fragment and an inactive haem domain. Cleavage can be prevented by using increased concentrations of peptone or certain amino acids such as Val or Leu Athelia rolfsii
heme the enzyme contains a protease-sensitive linker region, can be cleaved by endogenous proteases into a catalytically active flavin fragment and an inactive haem domain Athelia rolfsii