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Literature summary for 1.1.99.18 extracted from

  • Harreither, W.; Sygmund, C.; Augustin, M.; Narciso, M.; Rabinovich, M.L.; Gorton, L.; Haltrich, D.; Ludwig, R.
    Catalytic properties and classification of cellobiose dehydrogenases from Ascomycetes (2011), Appl. Environ. Microbiol., 77, 1804-1815.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.072
-
cellobiose at pH 6.0 and 30°C Amesia atrobrunnea
0.125
-
cellobiose at pH 5.0 and 30°C Dichomera saubinetii
0.14
-
cellobiose at pH 5.0 and 30°C Thermothelomyces fergusii
0.16
-
cellobiose at pH 5.0 and 30°C Hypoxylon haematostroma
0.16
-
cellobiose at pH 5.5 and 30°C Neurospora crassa
0.17
-
cellobiose at pH 5.5 and 30°C Stachybotrys bisbyi
0.185
-
lactose at pH 5.5 and 30°C Stachybotrys bisbyi
0.2
-
lactose at pH 5.0 and 30°C Thermothelomyces fergusii
0.215
-
lactose at pH 5.0 and 30°C Dichomera saubinetii
0.217
-
lactose at pH 6.0 and 30°C Amesia atrobrunnea
0.23
-
lactose at pH 5.0 and 30°C Hypoxylon haematostroma
0.325
-
lactose at pH 5.5 and 30°C Neurospora crassa
2.2
-
maltose at pH 5.0 and 30°C Thermothelomyces fergusii
8
-
maltose at pH 5.0 and 30°C Dichomera saubinetii
15.5
-
maltose at pH 6.0 and 30°C Amesia atrobrunnea
18
-
maltose at pH 5.0 and 30°C Hypoxylon haematostroma
50
-
maltose at pH 5.5 and 30°C Stachybotrys bisbyi
71
-
D-glucose at pH 5.0 and 30°C Hypoxylon haematostroma
79
-
D-glucose at pH 5.0 and 30°C Dichomera saubinetii
87
-
D-glucose at pH 6.0 and 30°C Amesia atrobrunnea
120
-
D-glucose at pH 5.0 and 30°C Thermothelomyces fergusii
120
-
maltose at pH 5.5 and 30°C Neurospora crassa
4000
-
D-glucose at pH 5.´5 and 30°C Neurospora crassa
17000
-
D-glucose at pH 5.´5 and 30°C Stachybotrys bisbyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81840
-
x * 81840, calculated from amino acid sequence Thermothelomyces fergusii
83587
-
x * 83587, calculated from amino acid sequence Neurospora crassa
83901
-
x * 83901, calculated from amino acid sequence Hypoxylon haematostroma
85000
-
x * 85000, SDS-PAGE Thermothelomyces fergusii
85000
-
x * 85000, SDS-PAGE Hypoxylon haematostroma
85942
-
x * 85942, calculated from amino acid sequence Amesia atrobrunnea
86688
-
x * 86688, calculated from amino acid sequence Stachybotrys bisbyi
86721
-
x * 86721, calculated from amino acid sequence Dichomera saubinetii
90000
-
x * 90000, SDS-PAGE Neurospora crassa
90000
-
x * 90000, SDS-PAGE Amesia atrobrunnea
100000
-
x * 100000, SDS-PAGE Stachybotrys bisbyi
115000
-
x * 115000, SDS-PAGE Dichomera saubinetii

Organism

Organism UniProt Comment Textmining
Amesia atrobrunnea E7D6C0
-
-
Dichomera saubinetii E7D6C1
-
-
Hypoxylon haematostroma
-
-
-
Neurospora crassa
-
-
-
Stachybotrys bisbyi E7D6C4
-
-
Thermothelomyces fergusii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and phenyl-Source column chromatography Neurospora crassa
DEAE-Sepharose column chromatography and phenyl-Source column chromatography Thermothelomyces fergusii
DEAE-Sepharose column chromatography and phenyl-Source column chromatography Stachybotrys bisbyi
DEAE-Sepharose column chromatography and phenyl-Source column chromatography Hypoxylon haematostroma
DEAE-Sepharose column chromatography and phenyl-Source column chromatography Amesia atrobrunnea
DEAE-Sepharose column chromatography and phenyl-Source column chromatography Dichomera saubinetii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
after 14.8fold purification, at pH 4.0 and 37°C Stachybotrys bisbyi
2.7
-
after 14.8fold purification, at pH 4.5 and 37°C Stachybotrys bisbyi
5.7
-
after 48.1fold purification, at pH 4.0 and 37°C Neurospora crassa
7.1
-
after 293fold purification, at pH 4.0 and 37°C Amesia atrobrunnea
7.4
-
after 21.2fold purification, at pH 4.0 and 37°C Hypoxylon haematostroma
8.9
-
after 7.3fold purification, at pH 4.0 and 37°C Thermothelomyces fergusii
9
-
after 21.2fold purification, at pH 5.0 and 37°C Hypoxylon haematostroma
10.3
-
after 48.1fold purification, at pH 5.5 and 37°C Neurospora crassa
11.5
-
after 293fold purification, at pH 6.0 and 37°C Amesia atrobrunnea
12.3
-
after 26.3fold purification, at pH 4.0 and 37°C Dichomera saubinetii
13.2
-
after 26.3fold purification, at pH 5.5 and 37°C Dichomera saubinetii
17.8
-
after 7.3fold purification, at pH 5.0 and 37°C Thermothelomyces fergusii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichloroindophenol
-
Neurospora crassa cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
cellobiose + 2,6-dichloroindophenol
-
Thermothelomyces fergusii cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
cellobiose + 2,6-dichloroindophenol
-
Stachybotrys bisbyi cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
cellobiose + 2,6-dichloroindophenol
-
Hypoxylon haematostroma cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
cellobiose + 2,6-dichloroindophenol
-
Amesia atrobrunnea cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
cellobiose + 2,6-dichloroindophenol
-
Dichomera saubinetii cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
D-glucose + 2,6-dichloroindophenol
-
Neurospora crassa D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
D-glucose + 2,6-dichloroindophenol
-
Thermothelomyces fergusii D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
D-glucose + 2,6-dichloroindophenol
-
Hypoxylon haematostroma D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
D-glucose + 2,6-dichloroindophenol
-
Amesia atrobrunnea D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
D-glucose + 2,6-dichloroindophenol
-
Dichomera saubinetii D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
Stachybotrys bisbyi D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
lactose + 2,6-dichloroindophenol
-
Neurospora crassa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
lactose + 2,6-dichloroindophenol
-
Thermothelomyces fergusii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
lactose + 2,6-dichloroindophenol
-
Stachybotrys bisbyi 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
lactose + 2,6-dichloroindophenol
-
Amesia atrobrunnea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
lactose + 2,6-dichloroindophenol
-
Dichomera saubinetii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
lactose + 2,6-dichloroindophenol
-
Hypoxylon haematostroma 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced 2,6dichloro-indophenol
-
?
lactose + cytochrome c
-
Neurospora crassa 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
?
lactose + cytochrome c
-
Thermothelomyces fergusii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
?
lactose + cytochrome c
-
Stachybotrys bisbyi 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
?
lactose + cytochrome c
-
Hypoxylon haematostroma 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
?
lactose + cytochrome c
-
Amesia atrobrunnea 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
?
lactose + cytochrome c
-
Dichomera saubinetii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced cytochrome c
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
-
Neurospora crassa 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
-
Thermothelomyces fergusii 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
-
Stachybotrys bisbyi 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
-
Hypoxylon haematostroma 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
-
Amesia atrobrunnea 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
-
Dichomera saubinetii 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE Neurospora crassa
? x * 90000, SDS-PAGE Amesia atrobrunnea
? x * 100000, SDS-PAGE Stachybotrys bisbyi
? x * 115000, SDS-PAGE Dichomera saubinetii
? x * 85000, SDS-PAGE Thermothelomyces fergusii
? x * 85000, SDS-PAGE Hypoxylon haematostroma
? x * 81840, calculated from amino acid sequence Thermothelomyces fergusii
? x * 83587, calculated from amino acid sequence Neurospora crassa
? x * 83901, calculated from amino acid sequence Hypoxylon haematostroma
? x * 85942, calculated from amino acid sequence Amesia atrobrunnea
? x * 86688, calculated from amino acid sequence Stachybotrys bisbyi
? x * 86721, calculated from amino acid sequence Dichomera saubinetii

Synonyms

Synonyms Comment Organism
Cdh
-
Neurospora crassa
Cdh
-
Thermothelomyces fergusii
Cdh
-
Stachybotrys bisbyi
Cdh
-
Hypoxylon haematostroma
Cdh
-
Amesia atrobrunnea
Cdh
-
Dichomera saubinetii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.51
-
maltose at pH 5.5 and 30°C Stachybotrys bisbyi
0.6
-
maltose at pH 5.5 and 30°C Neurospora crassa
1.38
-
maltose at pH 5.0 and 30°C Dichomera saubinetii
1.45
-
maltose at pH 5.0 and 30°C Hypoxylon haematostroma
1.8
-
maltose at pH 5.0 and 30°C Thermothelomyces fergusii
4
-
maltose at pH 6.0 and 30°C Amesia atrobrunnea
13
-
cellobiose at pH 5.0 and 30°C Thermothelomyces fergusii
16.8
-
D-glucose at pH 6.0 and 30°C Amesia atrobrunnea
18.4
-
D-glucose at pH 5.0 and 30°C Hypoxylon haematostroma
19.9
-
cellobiose at pH 5.5 and 30°C Stachybotrys bisbyi
20.1
-
cellobiose at pH 6.0 and 30°C Amesia atrobrunnea
20.2
-
lactose at pH 5.5 and 30°C Stachybotrys bisbyi
21.8
-
cellobiose at pH 5.0 and 30°C Hypoxylon haematostroma
23.6
-
D-glucose at pH 5.0 and 30°C Dichomera saubinetii
24.2
-
D-glucose at pH 5.5 and 30°C Stachybotrys bisbyi
24.7
-
lactose at pH 5.0 and 30°C Thermothelomyces fergusii
25
-
lactose at pH 5.0 and 30°C Hypoxylon haematostroma
25.6
-
D-glucose at pH 5.0 and 30°C Thermothelomyces fergusii
27.8
-
cellobiose at pH 5.0 and 30°C Dichomera saubinetii
28.9
-
lactose at pH 5.0 and 30°C Dichomera saubinetii
33.7
-
lactose at pH 6.0 and 30°C Amesia atrobrunnea
37.7
-
D-glucose at pH 5.5 and 30°C Neurospora crassa
51.8
-
lactose at pH 5.5 and 30°C Neurospora crassa
53.1
-
cellobiose at pH 5.5 and 30°C Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Stachybotrys bisbyi
5
-
-
Thermothelomyces fergusii
5
-
-
Hypoxylon haematostroma
5
-
-
Dichomera saubinetii
5.5
-
-
Neurospora crassa
6
-
-
Amesia atrobrunnea

pI Value

Organism Comment pI Value Maximum pI Value
Thermothelomyces fergusii isoelectric focusing
-
3.8
Amesia atrobrunnea isoelectric focusing
-
4.1
Dichomera saubinetii isoelectric focusing
-
4.2
Stachybotrys bisbyi isoelectric focusing
-
4.3
Hypoxylon haematostroma isoelectric focusing
-
4.3
Neurospora crassa isoelectric focusing
-
4.4
Thermothelomyces fergusii calculated from amino acid sequence
-
4.6
Amesia atrobrunnea calculated from amino acid sequence
-
5
Dichomera saubinetii calculated from amino acid sequence
-
5
Stachybotrys bisbyi calculated from amino acid sequence
-
5.1
Hypoxylon haematostroma calculated from amino acid sequence
-
5.7
Neurospora crassa calculated from amino acid sequence
-
6.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.005
-
maltose at pH 5.5 and 30°C Neurospora crassa
0.009
-
D-glucose at pH 5.5 and 30°C Neurospora crassa
0.01
-
maltose at pH 5.5 and 30°C Stachybotrys bisbyi
0.014
-
D-glucose at pH 5.5 and 30°C Stachybotrys bisbyi
0.08
-
maltose at pH 5.0 and 30°C Hypoxylon haematostroma
0.175
-
maltose at pH 5.0 and 30°C Dichomera saubinetii
0.195
-
D-glucose at pH 6.0 and 30°C Amesia atrobrunnea
0.215
-
D-glucose at pH 5.0 and 30°C Thermothelomyces fergusii
0.26
-
maltose at pH 6.0 and 30°C Amesia atrobrunnea
0.26
-
D-glucose at pH 5.0 and 30°C Hypoxylon haematostroma
0.3
-
D-glucose at pH 5.0 and 30°C Dichomera saubinetii
0.82
-
maltose at pH 5.0 and 30°C Thermothelomyces fergusii
93
-
cellobiose at pH 5.0 and 30°C Thermothelomyces fergusii
109
-
lactose at pH 5.0 and 30°C Hypoxylon haematostroma
109
-
lactose at pH 5.5 and 30°C Stachybotrys bisbyi
117
-
cellobiose at pH 5.5 and 30°C Stachybotrys bisbyi
124
-
lactose at pH 5.0 and 30°C Thermothelomyces fergusii
134
-
lactose at pH 5.0 and 30°C Dichomera saubinetii
136
-
cellobiose at pH 5.0 and 30°C Hypoxylon haematostroma
155
-
lactose at pH 6.0 and 30°C Amesia atrobrunnea
159
-
lactose at pH 5.5 and 30°C Neurospora crassa
222
-
cellobiose at pH 5.0 and 30°C Dichomera saubinetii
279
-
cellobiose at pH 6.0 and 30°C Amesia atrobrunnea
332
-
cellobiose at pH 5.5 and 30°C Neurospora crassa