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Literature summary for 1.1.99.18 extracted from

  • Sygmund, C.; Kracher, D.; Scheiblbrandner, S.; Zahma, K.; Felice, A.K.; Harreither, W.; Kittl, R.; Ludwig, R.
    Characterization of the two Neurospora crassa cellobiose dehydrogenases and their connection to oxidative cellulose degradation (2012), Appl. Environ. Microbiol., 78, 6161-6171.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X-33 Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
cellobiose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
0.043
-
cellopentaose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
0.045
-
cellotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
0.061
-
cellotetraose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
0.089
-
lactose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
0.196
-
cellopentaose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
0.204
-
cellotetraose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
0.23
-
cellotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
0.29
-
lactose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
0.81
-
xylotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
0.9
-
cellobiose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.2
-
maltotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.31
-
xylobiose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
3
-
xylotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
3.44
-
maltose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
3.6
-
xylobiose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
4.5
-
maltotetraose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
8.4
-
Mannopentaose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
17.2
-
maltose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
34.3
-
maltotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
35.7
-
maltotetraose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
39.2
-
Mannopentaose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
550
-
D-glucose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1545
-
D-xylose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
2770
-
D-xylose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
3600
-
D-galactose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
3700
-
D-glucose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
10000
-
D-mannose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
13300
-
D-mannose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
x * 85000, isoform CDH IIA, SDS-PAGE Neurospora crassa
85800
-
x * 85800, isoform CDH IIB, SDS-PAGE Neurospora crassa
110000 130000 gel filtration Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 1,4-benzoquinone
-
Neurospora crassa cellobiono-1,5-lactone + reduced 1,4-benzoquinone
-
?
cellobiose + 2,6-dichloroindophenol
-
Neurospora crassa cellobiono-1,5-lactone + reduced 2,6-dichloroindophenol
-
?
cellobiose + cytochrome c
-
Neurospora crassa cellobiono-1,5-lactone + reduced cytochrome c
-
?
cellopentaose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?
cellotetraose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?
cellotriose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?
cellulose + ferrocenium hexafluorophosphate
-
Neurospora crassa cellobiono-1,5-lactone + ferricenium hexafluorophosphate
-
?
D-galactose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?
D-glucose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?
D-mannose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?
D-xylose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?
lactose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?
maltose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?
maltotetraose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?
maltotriose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?
mannopentaose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?
additional information isoform CDH IIA exhibits 4 to 5times higher apparent catalytic constants (kcat and Km values) than isoform CDH IIB for most tested carbohydrates Neurospora crassa ?
-
?
xylobiose + 1,4-benzoquinone
-
Neurospora crassa 4-O-beta-D-xylopyranosyl-D-xylono-1,5-lactone + reduced 1,4-benzoquinone
-
?
xylotriose + 1,4-benzoquinone
-
Neurospora crassa ? + reduced 1,4-benzoquinone
-
?

Subunits

Subunits Comment Organism
? x * 85000, isoform CDH IIA, SDS-PAGE Neurospora crassa
? x * 85800, isoform CDH IIB, SDS-PAGE Neurospora crassa

Synonyms

Synonyms Comment Organism
CDH IIA isoform Neurospora crassa
CDH IIB isoform Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
maltose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1
-
maltotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.4
-
maltotetraose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.5
-
D-galactose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.5
-
D-xylose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.8
-
maltotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
1.9
-
D-xylose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
2.2
-
maltotetraose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
2.3
-
xylotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
3.5
-
maltose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
3.6
-
D-galactose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
4.3
-
D-mannose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
4.7
-
D-mannose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
5.8
-
xylobiose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
6
-
xylotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
7.4
-
Mannopentaose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
8.1
-
D-glucose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
10.2
-
cellopentaose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
11.4
-
cellobiose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
11.6
-
cellotetraose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
11.8
-
lactose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
12.4
-
cellotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
19.8
-
Mannopentaose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
45.4
-
xylobiose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
45.8
-
cellobiose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
46
-
cellotetraose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
46.6
-
cellopentaose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
49.3
-
lactose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
49.7
-
cellotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
55
-
D-glucose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
using 2,6-dichlorophenolindol as electron donor Neurospora crassa
7
-
using 1,4-benzoquinone as electron donor Neurospora crassa

pH Range

pH Minimum pH Maximum Comment Organism
3.7 8 more than 50% relative activity from pH 3.8 to 6.5 using 1,4-benzoquinone as electron acceptor Neurospora crassa
3.8 6.5 more than 50% relative activity from pH 3.8 to 6.5 using 2,6-dichlorophenolindol as electron acceptor Neurospora crassa

pI Value

Organism Comment pI Value Maximum pI Value
Neurospora crassa isoform CDH IIB, isoelectric focusing
-
4.9
Neurospora crassa isoform CDH IIA, isoelectric focusing
-
5.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0003
-
D-mannose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
0.0004
-
D-mannose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
0.0005
-
D-xylose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
0.0012
-
D-xylose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
0.014
-
D-glucose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
0.015
-
D-glucose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
0.023
-
maltose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
0.053
-
maltotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
0.061
-
maltotetraose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
0.506
-
Mannopentaose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
0.8
-
maltotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
0.88
-
Mannopentaose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
1.02
-
maltose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
2
-
xylotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
2.74
-
maltotetraose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
2.8
-
xylotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
4.45
-
xylobiose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
12.8
-
xylobiose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
133
-
lactose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
170
-
lactose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
189
-
cellotetraose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
216
-
cellotriose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
226
-
cellotetraose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
238
-
cellopentaose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
238
-
cellopentaose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
278
-
cellotriose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa
508
-
cellobiose isoform CDH IIA, at pH 6.0 and 30°C Neurospora crassa
527
-
cellobiose isoform CDH IIB, at pH 6.0 and 30°C Neurospora crassa