Cloned (Comment) | Organism |
---|---|
expressed in Aspergillus oryzae Jal355 cells | Thermothielavioides terrestris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
cellobiose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
0.0056 | - |
cellotetraose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
0.0075 | - |
cellopentaose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
0.0082 | - |
cellotriose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
0.023 | - |
lactose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
0.078 | - |
2,6-dichlorophenol-indophenol | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
2.5 | - |
maltose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
54 | - |
D-glucose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1000 | - |
D-xylose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | secretome | Thermothielavioides terrestris | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
125000 | - |
x * 125000, SDS-PAGE | Thermothielavioides terrestris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellobiose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris NRRL8126 | - |
cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellopentaose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellopentaose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris NRRL8126 | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellotetraose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellotetraose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris NRRL8126 | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellotriose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellotriose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris NRRL8126 | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
D-glucose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
D-xylose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
lactose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
maltose + 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | - |
? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
additional information | Thermothielavioides terrestris | when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24°C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol | ? | - |
? | |
additional information | Thermothielavioides terrestris NRRL8126 | when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24°C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothielavioides terrestris | - |
- |
- |
Thermothielavioides terrestris NRRL8126 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | approximately 40% of the expressed and processed protein represents glycosylation | Thermothielavioides terrestris |
Purification (Comment) | Organism |
---|---|
Mono Q column chromatography and Source 15PHE column chromatography | Thermothielavioides terrestris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.85 | - |
purified enzyme, with cellobiose as substrate, in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellobiose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris NRRL8126 | cellobiono-1,5-lactone + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellopentaose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellopentaose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris NRRL8126 | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellotetraose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellotetraose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris NRRL8126 | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellotriose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
cellotriose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris NRRL8126 | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
D-glucose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
D-xylose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
lactose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
maltose + 2,6-dichlorophenol-indophenol | - |
Thermothielavioides terrestris | ? + reduced 2,6-dichlorophenol-indophenol | - |
? | |
additional information | when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24°C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris | ? | - |
? | |
additional information | when incubated with insoluble microcrystalline cellulose (avicel) at pH 5.0 and ambient temperature (about 24°C), only 0.1% of the original enzyme activity remains in the supernatant as assayed with cellobiose and 2,6-dichlorophenol-indophenol | Thermothielavioides terrestris NRRL8126 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 125000, SDS-PAGE | Thermothielavioides terrestris |
Synonyms | Comment | Organism |
---|---|---|
cbdA | - |
Thermothielavioides terrestris |
Cdh | - |
Thermothielavioides terrestris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 87 | the enzyme unfolds over the range of 65-87°C, with a Tm value of 80.2°C | Thermothielavioides terrestris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
maltose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
0.33 | - |
D-xylose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.01 | - |
D-glucose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.23 | - |
cellobiose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.23 | - |
cellotetraose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.23 | - |
cellopentaose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.25 | - |
cellotriose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
1.4 | - |
lactose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
2.05 | - |
2,6-dichlorophenol-indophenol | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermothielavioides terrestris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | below pH 4.0, and particularly above pH 6.0, the activity is observed to drop (65% activity at pH 3.0, 45% activity at pH 7.0 and no activity at pH 9.0) | Thermothielavioides terrestris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Thermothielavioides terrestris | |
heme | - |
Thermothielavioides terrestris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00033 | - |
D-xylose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
0.019 | - |
D-glucose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
0.068 | - |
maltose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
62 | - |
lactose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
150 | - |
cellotriose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
160 | - |
cellopentaose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
220 | - |
cellotetraose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris | |
320 | - |
cellobiose | in 50 mM MES buffer (pH 6.0), at 25°C | Thermothielavioides terrestris |