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Literature summary for 1.1.99.18 extracted from

  • Zhang, R.; Xu, C.; Shen, Q.; Kasuga, T.; Wu, W.; Szewczyk, E.; Ma, D.; Fan, Z.
    Characterization of two cellobiose dehydrogenases and comparison of their contributions to total activity in Neurospora crassa (2013), Int. Biodeter. Biodegrad., 82, 24-32.
No PubMed abstract available
Search for more literature for 1.1.99.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Neurospora crassa strain 2489 Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00579
-
 cellobiose isoform CDH2, at pH 6.0 and 50°C Neurospora crassa
0.02572
-
 cellobiose isoform CDH1, at pH 6.0 and 50°C Neurospora crassa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Neurospora crassa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
 1 * 86000, mature peptides, calculated from amino acid sequence Neurospora crassa
95000
-
 1 * 95000, deglycosylated enzyme forms, SDS-PAGE Neurospora crassa
100000
-
 1 * 100000, isoform CDH1, SDS-PAGE Neurospora crassa
130000
-
 1 * 130000, isoform CDH2, SDS-PAGE Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-
Neurospora crassa FGSC 2489
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Neurospora crassa

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Sephacryl S-200 gel filtration Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:avicel-grown cell
-
 Neurospora crassa
-
culture condition:rice straw-grown cell
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.28
-
 isoform CDH2 from extracellular medium, at pH 6.0 and 50°C Neurospora crassa
1.19
-
 isoform CDH1 from extracellular medium, at pH 6.0 and 50°C Neurospora crassa
7.93
-
 isoform CDH2 after 28.32fold purification, at pH 6.0 and 50°C Neurospora crassa
8.89
-
 isoform CDH1 after 7.47fold purification, at pH 6.0 and 50°C Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + 2,6-dichlorophenol indophenol
-
 Neurospora crassa cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
cellobiose + 2,6-dichlorophenol indophenol
-
 Neurospora crassa FGSC 2489 cellobiono-1,5-lactone + reduced 2,6-dichlorophenol indophenol
-
 ?
additional information isoform CDH2 lacks the cellulose-binding module and contributes marginally to total enzyme activity but has specific activity similar to that of isoform CDH1 and a much lower Km value Neurospora crassa ?
-
 ?
additional information isoform CDH2 lacks the cellulose-binding module and contributes marginally to total enzyme activity but has specific activity similar to that of isoform CDH1 and a much lower Km value Neurospora crassa FGSC 2489 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, isoform CDH1, SDS-PAGE Neurospora crassa
monomer 1 * 130000, isoform CDH2, SDS-PAGE Neurospora crassa
monomer 1 * 86000, mature peptides, calculated from amino acid sequence Neurospora crassa
monomer 1 * 95000, deglycosylated enzyme forms, SDS-PAGE Neurospora crassa

Synonyms

Synonyms Comment Organism
CDH1 isoform Neurospora crassa
Cdh2 isoform Neurospora crassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Neurospora crassa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 55 about 70% activity between 45 and 55°C Neurospora crassa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 30 the enzymes are stable for 10 h at 20-30°C Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.7
-
 cellobiose isoform CDH2, at pH 6.0 and 50°C Neurospora crassa
13.5
-
 cellobiose isoform CDH1, at pH 6.0 and 50°C Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Neurospora crassa

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 about 50% activity between pH 5.0 and 7.5 Neurospora crassa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 enzyme activities decrease sharply when stored in buffers with pHs below 3.0 or above 8.0 Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
flavin
-
 Neurospora crassa
heme
-
 Neurospora crassa

Expression

Organism Comment Expression
Neurospora crassa the enzyme is produced when avicel or rice straw is used as the carbon source up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
525
-
 cellobiose isoform CDH1, at pH 6.0 and 50°C Neurospora crassa
2193
-
 cellobiose isoform CDH2, at pH 6.0 and 50°C Neurospora crassa