Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetate | 50 mM: activates, 100 mM: 100% increase in activity at pH 4 | Agaricus bisporus |
General Stability | Organism |
---|---|
freezing in 50 mM sodium phosphate, 2.4 mg/ml, results in 90% loss of activity | Agaricus bisporus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 10 mM, 100% inhibition | Agaricus bisporus | |
CN- | 10 mM, 83% inhibition | Agaricus bisporus | |
Cu2+ | 10 mM, 90% inhibition | Agaricus bisporus | |
HgCl2 | 10 mM, 100% inhibition | Agaricus bisporus | |
additional information | not inhibited by Ca2+, Co2+, Fe2+, Fe3+, Mg2+, Mn2+, NH4+, Zn2+, F-, EDTA, NaN3, dithiothreitol, phenanthroline and H2O2, each at 10 mM | Agaricus bisporus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | enzyme is actively secreted into the extracellular fluid | Agaricus bisporus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
1 * 75000, SDS-PAGE | Agaricus bisporus |
79000 | - |
gel filtration | Agaricus bisporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Agaricus bisporus | oxidative carbohydrate metabolism, pyranose 2-dehydrogenase may play an important role in fungal lignocellulose decomposition by interconnecting ligninolysis with degradation of cell wall polysaccharide components | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agaricus bisporus | - |
- |
- |
Agaricus bisporus | - |
hybrid strain U3 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Agaricus bisporus |
Purification (Comment) | Organism |
---|---|
68.3fold | Agaricus bisporus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a pyranoside + acceptor = a pyranosid-3-ulose (or a pyranosid-3,4-diulose) + reduced acceptor | requires FAD, a number of aldoses and ketoses in pyranose form, as well as glycosides, gluco-oligosaccharides, sucrose and lactose can act as donor, 1,4-benzoquinone or ferricenium ion (ferrocene oxidized by removal of one electron) can serve as acceptor, unlike EC 1.1.3.10, pyranose oxidase, this fungal enzyme does not interact with O2 and exhibits extremely broad substrate tolerance with variable regioselectivity (C-3, C-2 or C3 + C-2 or C-3 + C-4) for (di)oxidation of different sugars, D-glucose is exclusively or preferentially oxidized at C-3 (depending on the enzyme source), but can also be oxidized at C-2 + C-3, the enzyme also acts on 1-4-alpha- and 1-4-beta-gluco-oligosaccharides, non-reducing gluco-oligosaccharides and L-arabinose, which are not substrates of EC 1.1.3.10, sugars are oxidized in their pyranose but not in their furanose form | Agaricus bisporus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | 17 days old | Agaricus bisporus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.4 | - |
pH 6.5, 25°C | Agaricus bisporus |
Storage Stability | Organism |
---|---|
4°C, purified enzyme, 100 mM sodium phosphate, pH 6, 7, 8 or 9, 100 days, stable | Agaricus bisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a pyranoside + acceptor | - |
Agaricus bisporus | a 3-dehydropyranoside (or 3,4-didehydropyranoside) + reduced acceptor | - |
? | |
aldopyranose + 1,4-benzoquinone | more active as electron acceptor than 2,6-dichlorophenolindophenol or ferricyanide, less active than 3,5-di-tert-butyl-1,2-benzoquinone, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses | Agaricus bisporus | 2-aldoketose + hydroquinone | - |
? | |
aldopyranose + 2,6-dichlorophenolindophenol | 65.9% of the activity with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses | Agaricus bisporus | 2-aldoketose + reduced 2,6-dichlorophenolindophenol | - |
? | |
aldopyranose + 3,5-di-tert-butyl-1,2-benzoquinone | 4.3fold higher activity than with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses | Agaricus bisporus | 2-aldoketose + reduced 3,5-di-tert-butyl-1,2-benzoquinone | - |
? | |
aldopyranose + ferricyanide | 10.4% of the activity with 1,4-benzoquinone as electron acceptor, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses | Agaricus bisporus | 2-aldoketose + ferrocyanide | - |
? | |
aldopyranose + quinone | C-2 specific sugar oxidoreductase, highly nonspecific, several oligosaccharides and glycopyranosides are converted to the corresponding 2-aldoketoses/aldosuloses | Agaricus bisporus | 2-aldoketose + hydroquinone | - |
? | |
cellobiose + 1,4-benzoquinone | 94% of the activity with D-glucose | Agaricus bisporus | ? | - |
? | |
cellotriose + 1,4-benzoquinone | 56% of the activity with D-glucose | Agaricus bisporus | ? | - |
? | |
D-allose + 1,4-benzoquinone | 30% of the activity with D-glucose | Agaricus bisporus | 2-dehydro-D-allose + hydroquinone | - |
? | |
D-arabino-2-hexosulose + 1,4-benzoquinone | 59% of the activity with D-glucose | Agaricus bisporus | ? | - |
? | |
D-arabinose + 1,4-benzoquinone | 18% of the activity with D-glucose | Agaricus bisporus | 2-dehydro-D-arabinose + hydroquinone | - |
? | |
D-galactose + 1,4-benzoquinone | 88% of the activity with D-glucose | Agaricus bisporus | 2-dehydro-D-galactose + hydroquinone | - |
? | |
D-glucono-1,5-lactone + 1,4-benzoquinone | 118% of the activity with D-glucose | Agaricus bisporus | ? | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Agaricus bisporus | 2-dehydro-D-glucose + hydroquinone | D-arabino-2-hexosulose, 2-ketoglucose | ? | |
D-ribose + 1,4-benzoquinone | 34% of the activity with D-glucose | Agaricus bisporus | 2-dehydro-D-ribose + hydroquinone | - |
? | |
D-xylose + 1,4-benzoquinone | 116% of the activity with D-glucose | Agaricus bisporus | 2-dehydro-D-xylose + hydroquinone | - |
? | |
L-arabinose + 1,4-benzoquinone | 113% of the activity with D-glucose | Agaricus bisporus | 2-dehydro-L-arabinose + hydroquinone | - |
? | |
L-glucose + 1,4-benzoquinone | 61% of the activity with D-glucose | Agaricus bisporus | 2-dehydro-L-glucose + hydroquinone | - |
? | |
L-sorbose + 1,4-benzoquinone | 10% of the activity with D-glucose | Agaricus bisporus | 2-dehydro-L-sorbose + hydroquinone | - |
? | |
lactose + 1,4-benzoquinone | 21% of the activity with D-glucose | Agaricus bisporus | ? | - |
? | |
maltose + 1,4-benzoquinone | 93% of the activity with D-glucose | Agaricus bisporus | ? | - |
? | |
maltotriose + 1,4-benzoquinone | 70% of the activity with D-glucose | Agaricus bisporus | ? | - |
? | |
methyl-alpha-D-galactopyranoside + 1,4-benzoquinone | 23% of the activity with D-glucose | Agaricus bisporus | methyl-alpha-3-dehydro-D-galactopyranoside + hydroquinone | - |
? | |
methyl-alpha-D-glucopyranoside + 1,4-benzoquinone | 117% of the activity with D-glucose | Agaricus bisporus | methyl-alpha-3-dehydro-D-glucopyranoside + hydroquinone | - |
? | |
methyl-beta-D-glucopyranoside + 1,4-benzoquinone | 69% of the activity with D-glucose | Agaricus bisporus | methyl-beta-3-dehydro-D-glucopyranoside + hydroquinone | - |
? | |
additional information | oxidative carbohydrate metabolism, pyranose 2-dehydrogenase may play an important role in fungal lignocellulose decomposition by interconnecting ligninolysis with degradation of cell wall polysaccharide components | Agaricus bisporus | ? | - |
? | |
additional information | enzyme is incapable of reducing O2 to H2O2, not: cytochrome c, NAD(P)+, nitroblue tetrazolium, low or no activity with D-altrose, D-fructose, D-lyxose, D-mannose, D-arabinitol, D-glucitol, D-mannitol or erythritol | Agaricus bisporus | ? | - |
? | |
pyranose + acceptor | - |
Agaricus bisporus | 2-dehydropyranose (or 3-dehydropyranose or 2,3-didehydropyranose) + reduced acceptor | - |
? | |
sucrose + 1,4-benzoquinone | 63% of the activity with D-glucose | Agaricus bisporus | ? | - |
? | |
trehalose + 1,4-benzoquinone | 54% of the activity with D-glucose | Agaricus bisporus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 75000, SDS-PAGE | Agaricus bisporus |
Synonyms | Comment | Organism |
---|---|---|
dehydrogenase, pyranose 2/3- | - |
Agaricus bisporus |
pyranose 2-dehydrogenase | - |
Agaricus bisporus |
pyranose 2/3-dehydrogenase | - |
Agaricus bisporus |
pyranose 3-dehydrogenase | - |
Agaricus bisporus |
pyranose:quinone acceptor 2-oxidoreductase | - |
Agaricus bisporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Agaricus bisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
acetate buffer, two pH-optima | Agaricus bisporus |
9 | - |
phosphate buffer, two pH-optima | Agaricus bisporus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9.5 | activity in the range is higher than 50% of the rate at the acidic optimum at pH 4 | Agaricus bisporus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | contains a flavin prosthetic group | Agaricus bisporus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Agaricus bisporus | analytical isoelectric focusing | - |
4.2 |