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Literature summary for 1.1.99.29 extracted from

  • Sygmund, C.; Kittl, R.; Volc, J.; Halada, P.; Kubatova, E.; Haltrich, D.; Peterbauer, C.K.
    Characterization of pyranose dehydrogenase from Agaricus meleagris and its application in the C-2 specific conversion of D-galactose (2008), J. Biotechnol., 133, 334-342.
    View publication on PubMed
Search for more literature for 1.1.99.29

Activating Compound

Activating Compound Comment Organism Structure
NH4+ at 0.1-1 M in 20 mM sodium phosphate buffer pH 7.0, not due to increased ionic strenth Leucoagaricus meleagris

Application

Application Comment Organism
degradation lignocellulose degradation Leucoagaricus meleagris

Inhibitors

Inhibitors Comment Organism Structure
alpha,alpha-dipyridyl no activity Leucoagaricus meleagris
CN- no activity Leucoagaricus meleagris
EDTA no activity Leucoagaricus meleagris
F- no activity Leucoagaricus meleagris
MoO42- no activity Leucoagaricus meleagris
NaN3 no activity Leucoagaricus meleagris
NH4+ at 2 M in 20 mM sodium phosphate buffer pH 7.0 Leucoagaricus meleagris
p-hydroxymercuribenzoate no activity Leucoagaricus meleagris
Phenanthroline no activity Leucoagaricus meleagris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 Fe3+ at 30°C with 50 mM glucose as susbtrate Leucoagaricus meleagris
0.18
-
 methyl-1,4-benzoquinone at 30°C with 50 mM glucose as susbtrate Leucoagaricus meleagris
0.22
-
 3,5-di-tert-butyl-1,4-benzoquinone at 30°C with 50 mM glucose as susbtrate Leucoagaricus meleagris
0.54
-
 L-arabinose Fe3+ as electron acceptor Leucoagaricus meleagris
0.55
-
 2-chloro-1,4-benzoquinone at 30°C with 50 mM glucose as susbtrate Leucoagaricus meleagris
0.82
-
 D-glucose Fe3+ as electron acceptor Leucoagaricus meleagris
0.92
-
 2,5-chloro-1,4-benzoquinone at 30°C with 50 mM glucose as susbtrate Leucoagaricus meleagris
1.05
-
 D-galactose Fe3+ as electron acceptor Leucoagaricus meleagris
1.82
-
 1,4-benzoquinone at 30°C with 50 mM glucose as susbtrate Leucoagaricus meleagris
1.93
-
 D-xylose Fe3+ as electron acceptor Leucoagaricus meleagris
6.82
-
 cellobiose Fe3+ as electron acceptor Leucoagaricus meleagris
8.61
-
 D-maltose Fe3+ as electron acceptor Leucoagaricus meleagris
10.8
-
 D-xylobiose Fe3+ as electron acceptor Leucoagaricus meleagris
59.7
-
 D-ribose Fe3+ as electron acceptor Leucoagaricus meleagris
79.1
-
 D-talose Fe3+ as electron acceptor Leucoagaricus meleagris
108
-
 D-mannose Fe3+ as electron acceptor Leucoagaricus meleagris
134
-
 lactose Fe3+ as electron acceptor Leucoagaricus meleagris
137
-
 L-sorbose Fe3+ as electron acceptor Leucoagaricus meleagris
153
-
 D-fructose Fe3+ as electron acceptor Leucoagaricus meleagris
156
-
 maltotriose Fe3+ as electron acceptor Leucoagaricus meleagris

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Leucoagaricus meleagris
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ 37% inhibition of activity Leucoagaricus meleagris
Al3+ no activity Leucoagaricus meleagris
Ca2+ no activity Leucoagaricus meleagris
Cu2+ no activity Leucoagaricus meleagris
Fe2+ no activity Leucoagaricus meleagris
Fe3+ 39% inhibition of activity Leucoagaricus meleagris
Hg2+ 25% inhibition of activity Leucoagaricus meleagris
Mg2+ no activity Leucoagaricus meleagris
Mn2+ no activity Leucoagaricus meleagris
Ni2+ no activity Leucoagaricus meleagris
Zn2+ no activity Leucoagaricus meleagris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 SDS-PAGE, PDHf, second band Leucoagaricus meleagris
66500
-
 SDS-PAGE, gel filtration, MALDI MS Leucoagaricus meleagris

Organism

Organism UniProt Comment Textmining
Leucoagaricus meleagris
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content of 7% Leucoagaricus meleagris

Purification (Commentary)

Purification (Comment) Organism
using DEAE-Sephacel anion exchange chromatography and FPLC on phenyl-superose HR 10/10, Mono Q HR 5/5 and superose 12 HR 30/10 chromatography Leucoagaricus meleagris

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
stable for 3 months at 4°C, in sodium phosphate buffer pH 7.0 Leucoagaricus meleagris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + Fe3+
-
 Leucoagaricus meleagris ?
-
 ?
D-fructose + Fe3+
-
 Leucoagaricus meleagris ?
-
 ?
D-galactose + benzoquinone
-
 Leucoagaricus meleagris 2-dehydro-D-galactose + hydroquinone no 2,3-didehydro-d-galactose is detectable ?
D-galactose + Fe3+
-
 Leucoagaricus meleagris 2-dehydro-D-galactose + Fe2+ + H+
-
 ?
D-glucose pH 8.5 Leucoagaricus meleagris 3-dehydro-D-glucose + Fe2+ + H+
-
 ?
D-glucose + 2,5-chloro-1,4-benzoquinone pH 5 Leucoagaricus meleagris ?
-
 ?
D-glucose + 2,6-dichloroindophenol pH 4 Leucoagaricus meleagris ?
-
 ?
D-glucose + 2-chloro-1,4-benzoquinone pH 5 Leucoagaricus meleagris ?
-
 ?
D-glucose + 3,5-di-tert-butyl-1,4-benzoquinone pH 6 Leucoagaricus meleagris ?
-
 ?
D-glucose + benzoquinone pH 3 Leucoagaricus meleagris 2,3-didehydro-D-glucose + hydroquinone
-
 ?
D-glucose + methyl-1,4-benzoquinone pH 8 Leucoagaricus meleagris ?
-
 ?
D-maltose + Fe3+
-
 Leucoagaricus meleagris ?
-
 ?
D-maltotriose + Fe3+
-
 Leucoagaricus meleagris ?
-
 ?
D-mannose + Fe3+
-
 Leucoagaricus meleagris ?
-
 ?
D-ribose + Fe3+
-
 Leucoagaricus meleagris ?
-
 ?
D-talose + Fe3+
-
 Leucoagaricus meleagris ?
-
 ?
D-xylobiose + ferricenium ion
-
 Leucoagaricus meleagris ?
-
 r
D-xylose + Fe3+
-
 Leucoagaricus meleagris ?
-
 ?
L-arabinose + Fe3+
-
 Leucoagaricus meleagris ?
-
 ?
L-sorbose + Fe3+
-
 Leucoagaricus meleagris ?
-
 ?
L-xylobiose + Fe3+
-
 Leucoagaricus meleagris ?
-
 ?
lactose + Fe3+
-
 Leucoagaricus meleagris ?
-
 ?
maltotriose + ferricenium ion
-
 Leucoagaricus meleagris ?
-
 r

Subunits

Subunits Comment Organism
monomer gel filtration Leucoagaricus meleagris

Synonyms

Synonyms Comment Organism
PDH
-
 Leucoagaricus meleagris
pyranose dehydrogenase
-
 Leucoagaricus meleagris
pyranose:acceptor oxidoreductase
-
 Leucoagaricus meleagris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
63
-
-
 Leucoagaricus meleagris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 50% activity loss after incubation at 50°C for 50 h Leucoagaricus meleagris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
 for glucose and 1,4-benzoquinone as substrates Leucoagaricus meleagris
9
-
 Fe3+ as electron acceptor, with bisTris propane buffer Leucoagaricus meleagris

pH Range

pH Minimum pH Maximum Comment Organism
2 10 depending on the electron acceptor and with d-glucose as electron donor, has 2 pH optima for some substrates Leucoagaricus meleagris

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
 80% maximum stability at pH 7 Leucoagaricus meleagris

Cofactor

Cofactor Comment Organism Structure
FAD 5 FAD covalently bound Leucoagaricus meleagris

pI Value

Organism Comment pI Value Maximum pI Value
Leucoagaricus meleagris isoelectric focusing, 4 isoforms
-
 4.2
Leucoagaricus meleagris isoelectric focusing, 4 isoforms
-
 4.25
Leucoagaricus meleagris isoelectric focusing, 4 isoforms
-
 4.35
Leucoagaricus meleagris isoelectric focusing, 4 isoforms
-
 4.45