Cloned (Comment) | Organism |
---|---|
expressed in Aspergillus nidulans and Aspergillus niger | Leucoagaricus meleagris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
ferricenium ion | native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5 | Leucoagaricus meleagris | |
0.22 | - |
ferricenium ion | recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5 | Leucoagaricus meleagris | |
0.44 | - |
L-arabinose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
0.55 | - |
2-chloro-1,4-benzoquinone | native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0 | Leucoagaricus meleagris | |
0.61 | - |
2-chloro-1,4-benzoquinone | recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0 | Leucoagaricus meleagris | |
0.74 | - |
L-arabinose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
0.82 | - |
D-glucose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
0.86 | - |
D-glucose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
1.02 | - |
D-galactose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
1.03 | - |
D-xylose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
1.03 | - |
1,4-benzoquinone | recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0 | Leucoagaricus meleagris | |
1.05 | - |
D-galactose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
1.82 | - |
1,4-benzoquinone | native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0 | Leucoagaricus meleagris | |
1.93 | - |
D-xylose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
113.9 | - |
lactose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
134 | - |
lactose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
SDS-PAGE | Leucoagaricus meleagris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucoagaricus meleagris | Q3L245 | - |
- |
Leucoagaricus meleagris CCBAS 907 | Q3L245 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant protein contains approximately 7% N-linked carbohydrate moiety | Leucoagaricus meleagris |
Purification (Comment) | Organism |
---|---|
EMD DEAE 650 S column chromatography and Source 15 PHE column chromatography | Leucoagaricus meleagris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the recombinant enzyme from Aspergillus nidulans crude extract shows a specific activity of 13.5 units/mg using D-glucose as substrate, at 30°C in phosphate buffer, pH 7.5. The recombinant 4.1fold purified enzyme from Aspergillus nidulans shows a specific activity of 55.9 units/mg using D-glucose as substrate, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + ferricenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? | |
D-galactose + ferricenium ion | - |
Leucoagaricus meleagris CCBAS 907 | ? + ferrocene | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Leucoagaricus meleagris | ? | - |
? | |
D-glucose + 1,4-benzoquinone | - |
Leucoagaricus meleagris CCBAS 907 | ? | - |
? | |
D-glucose + 2-chloro-1,4-benzoquinone | - |
Leucoagaricus meleagris | ? | - |
? | |
D-glucose + 2-chloro-1,4-benzoquinone | - |
Leucoagaricus meleagris CCBAS 907 | ? | - |
? | |
D-glucose + ferricenium ion | - |
Leucoagaricus meleagris | 3-dehydro-D-glucose + ferrocene | - |
? | |
D-glucose + ferricenium ion | - |
Leucoagaricus meleagris CCBAS 907 | 3-dehydro-D-glucose + ferrocene | - |
? | |
D-xylose + ferricenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? | |
D-xylose + ferricenium ion | - |
Leucoagaricus meleagris CCBAS 907 | ? + ferrocene | - |
? | |
L-arabinose + ferricenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? | |
lactose + ferricenium ion | - |
Leucoagaricus meleagris | ? + ferrocene | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Leucoagaricus meleagris |
pyranose:acceptor oxidoreductase | - |
Leucoagaricus meleagris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.9 | - |
2-chloro-1,4-benzoquinone | recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0 | Leucoagaricus meleagris | |
15.1 | - |
2-chloro-1,4-benzoquinone | native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0 | Leucoagaricus meleagris | |
31.9 | - |
lactose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
37.2 | - |
L-arabinose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
39.6 | - |
lactose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
41.5 | - |
D-xylose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
43.4 | - |
D-xylose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
45.7 | - |
L-arabinose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
45.9 | - |
D-glucose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
46.6 | - |
D-galactose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
48.06 | - |
D-glucose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
48.5 | - |
D-galactose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
59 | - |
1,4-benzoquinone | recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0 | Leucoagaricus meleagris | |
76 | - |
1,4-benzoquinone | native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0 | Leucoagaricus meleagris | |
104 | - |
ferricenium ion | native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5 | Leucoagaricus meleagris | |
203 | - |
ferricenium ion | recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5 | Leucoagaricus meleagris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
using 1,4-benzoquinone as electron acceptor | Leucoagaricus meleagris |
5 | - |
using 2-chloro-1,4-benzoquinone as electron acceptor | Leucoagaricus meleagris |
8.5 | - |
using ferrocenium ion as electron acceptor | Leucoagaricus meleagris |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Leucoagaricus meleagris | isoelectric focusing | 4.7 | 4.2 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
lactose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
0.29 | - |
lactose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
20.9 | - |
2-chloro-1,4-benzoquinone | recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0 | Leucoagaricus meleagris | |
22.9 | - |
D-xylose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
25.5 | - |
2-chloro-1,4-benzoquinone | native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 5.0 | Leucoagaricus meleagris | |
40.3 | - |
D-xylose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
45.7 | - |
D-galactose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
46.2 | - |
D-galactose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
55.9 | - |
D-glucose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
57.3 | - |
1,4-benzoquinone | recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0 | Leucoagaricus meleagris | |
57.5 | - |
D-glucose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
57.5 | - |
1,4-benzoquinone | native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 2.0 | Leucoagaricus meleagris | |
61.8 | - |
L-arabinose | recombinant enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
62.1 | - |
L-arabinose | native enzyme, with 0.4 mM ferricenium ion as the electron acceptor, at 30°C in phosphate buffer, pH 7.5 | Leucoagaricus meleagris | |
802 | - |
ferricenium ion | native enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5 | Leucoagaricus meleagris | |
923 | - |
ferricenium ion | recombinant enzyme, with 25 mM D-glucose as cosubstrate, at 30°C in phosphate buffer, pH 8.5 | Leucoagaricus meleagris |