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Literature summary for 1.1.99.3 extracted from

  • Shinagawa, E.; Matsushita, K.; Adachi, O.; Ameyama, M.
    D-Gluconate dehydrogenase, 2-keto-D-gluconate yielding, from Gluconobacter dioxyacetonicus: purification and characterization (1984), Agric. Biol. Chem., 48, 1517-1522.
No PubMed abstract available
Search for more literature for 1.1.99.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 D-gluconate
-
 Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 1 * 21000 + 1 * 45000 + 1 * 64000, SDS-PAGE Gluconobacter oxydans
45000
-
 1 * 21000 + 1 * 45000 + 1 * 64000, SDS-PAGE Gluconobacter oxydans
64000
-
 1 * 21000 + 1 * 45000 + 1 * 64000, SDS-PAGE Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
237
-
-
 Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-gluconate + ferricyanide
-
 Gluconobacter oxydans 2-oxogluconate + ferrocyanide
-
 ?
D-gluconate + phenazine methosulfate
-
 Gluconobacter oxydans 2-oxogluconate + ?
-
 ?

Subunits

Subunits Comment Organism
trimer 1 * 21000 + 1 * 45000 + 1 * 64000, SDS-PAGE Gluconobacter oxydans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
flavin contains covalently bound flavin Gluconobacter oxydans
heme enzyme contains 2 mol of heme per mol of enzyme Gluconobacter oxydans