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Literature summary for 1.1.99.35 extracted from

  • Igarashi, S.; Sode, K.
    Construction and characterization of heterodimeric soluble quinoprotein glucose dehydrogenase (2004), J. Biochem. Biophys. Methods, 61, 331-338.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Acinetobacter calcoaceticus

Protein Variants

Protein Variants Comment Organism
additional information constructed of heterodimeric PQQGDH-B composed of native wild-type and inactive mutant H168Q subunits. The heterodimeric wild-type/H168Q shows slightly decreased GDH activity and almost identical substrate specificity profile to the wild-type enzyme. The Hill coefficient of the heterodimer is calculated as 1.13, indicating positive cooperativity Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 D-glucose recombinant wild-type with R4-tail, pH 7.0 Acinetobacter calcoaceticus
2 3 3-O-methyl-D-glucose recombinant wild-type with R4-tail, pH 7.0 Acinetobacter calcoaceticus
8
-
D-galactose recombinant wild-type with R4-tail, pH 7.0 Acinetobacter calcoaceticus
9
-
D-galactose heterodimer wild-type/mutant H168Q, pH 7.0 Acinetobacter calcoaceticus
13
-
maltose heterodimer wild-type/mutant H168Q, pH 7.0 Acinetobacter calcoaceticus
14
-
maltose recombinant wild-type with R4-tail, pH 7.0 Acinetobacter calcoaceticus
20
-
lactose heterodimer wild-type/mutant H168Q, pH 7.0 Acinetobacter calcoaceticus
20
-
lactose recombinant wild-type with R4-tail, pH 7.0 Acinetobacter calcoaceticus
22
-
D-glucose heterodimer wild-type/mutant H168Q, pH 7.0 Acinetobacter calcoaceticus
22
-
3-O-methyl-D-glucose heterodimer wild-type/mutant H168Q, pH 7.0 Acinetobacter calcoaceticus
34
-
D-allose recombinant wild-type with R4-tail, pH 7.0 Acinetobacter calcoaceticus
36
-
D-allose heterodimer wild-type/mutant H168Q, pH 7.0 Acinetobacter calcoaceticus

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-allose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-galactose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
D-glucose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?
lactose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol
-
?
maltose + oxidized 2,6-dichlorophenolindophenol
-
Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
dimer study on heterodimeric PQQGDH-B composed of native wild-type and inactive mutant H168Q subunits Acinetobacter calcoaceticus