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Literature summary for 1.10.3.1 extracted from

  • Paul, B.; Gowda, L.R.
    Purification and characterization of a polyphenol oxidase from the seeds of field bean (Dolichos lablab) (2000), J. Agric. Food Chem., 48, 3839-3846.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-hydroxy-2,4,6-cycloheptatrien-1-one trivial name tropolone Lablab purpureus
ascorbic acid
-
Lablab purpureus
cysteine
-
Lablab purpureus
Metabisulfite
-
Lablab purpureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18
-
L-Dopa
-
Lablab purpureus
4
-
4-methylcatechol
-
Lablab purpureus
10.5
-
catechol
-
Lablab purpureus
12.5
-
pyrogallol
-
Lablab purpureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
4 * 30000, SDS-PAGE Lablab purpureus
120000
-
gel filtration Lablab purpureus

Organism

Organism UniProt Comment Textmining
Lablab purpureus
-
field bean
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sephacel, Phenyl-agarose, Sephadex G-100 Lablab purpureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
68077.0 units/mg, 1 unit is defined as absorbance change of 0.001 per min at 25°C Lablab purpureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + O2 140% of activity with catechol Lablab purpureus 4-methyl-1,2-benzoquinone
-
?
catechol + 1/2 O2
-
Lablab purpureus 1,2-benzoquinone + H2O
-
?
L-dopa + 1/2 O2 22.65 of activity with catechol Lablab purpureus L-dopaquinone + H2O
-
?
pyrogallol + O2 24% of activity with catechol Lablab purpureus ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 30000, SDS-PAGE Lablab purpureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
catechol oxidation Lablab purpureus

pH Range

pH Minimum pH Maximum Comment Organism
3 5.5 50% activity at pH 3.5 and pH 5.5, 25% activity at pH 3.0 Lablab purpureus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00058
-
tropolone
-
Lablab purpureus
0.0055
-
Metabisulfite
-
Lablab purpureus
0.0075
-
ascorbic acid
-
Lablab purpureus
19.5
-
cysteine
-
Lablab purpureus