Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cinnamic acid | - |
Coffea arabica | |
hexadecyltrimethyl-ammonium bromide | - |
Coffea arabica | |
polyvinylpyrrolidone 40 | - |
Coffea arabica | |
Salicylhydroxamic acid | - |
Coffea arabica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.88 | - |
chlorogenic acid | leaf enzyme | Coffea arabica | |
2.27 | - |
chlorogenic acid | endosperm enzyme | Coffea arabica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorogenic acid + O2 | Coffea arabica | probably the major substrate in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coffea arabica | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | endosperm | Coffea arabica | - |
leaf | - |
Coffea arabica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylcatechol + O2 | - |
Coffea arabica | 4-methyl-1,2-benzoquinone | - |
? | |
chlorogenic acid + O2 | best substrate for both leaf and endosperm enzyme | Coffea arabica | ? | - |
? | |
chlorogenic acid + O2 | probably the major substrate in vivo | Coffea arabica | ? | - |
? | |
L-dopa + 1/2 O2 | endosperm enzyme, 3.9% of activity with chlorogenic acid | Coffea arabica | L-dopaquinone + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme may be synthesized as a 67000 Da precursor that can be hydrolyzed to a 45000 Da form still retaining catalytic activity | Coffea arabica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | leaf and endosperm enzyme | Coffea arabica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
leaf enzyme, no loss of activity after 30 min, 50% loss of activity after 5 min at 80°C, endosperm enzyme, 20% loss of activity after 30 min at 50°C, 50% loss of activity after 2.5 min at 80°C | Coffea arabica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | leaf and endosperm enzyme | Coffea arabica |