Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | 170% activity at 10 mM | Lonicera japonica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | nearly complete inhibition at 10 mM | Lonicera japonica | |
Citric acid | 32% residual activity at 10 mM | Lonicera japonica | |
Co2+ | 35% residual activity at 10 mM | Lonicera japonica | |
Cu2+ | 33% residual activity at 10 mM | Lonicera japonica | |
kojic acid | 11% residual activity at 10 mM | Lonicera japonica | |
Mg2+ | inhibits activity at 0.01 mM | Lonicera japonica | |
Mn2+ | inhibits activity at 0.01 mM | Lonicera japonica | |
additional information | not inhibited by SDS | Lonicera japonica | |
Sodium metabisulfite | 27% residual activity at 10 mM | Lonicera japonica | |
tropolone | 34% residual activity at 10 mM | Lonicera japonica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.213 | - |
catechol | at pH 7.5 and 25°C | Lonicera japonica | |
1 | - |
chlorogenic acid | at pH 7.5 and 25°C | Lonicera japonica | |
2.69 | - |
L-Dopa | at pH 7.5 and 25°C | Lonicera japonica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 124% activity at 10 mM | Lonicera japonica | |
copper | a copper-containing enzyme with a dinuclear copper centre | Lonicera japonica | |
Mg2+ | stimulates activity to 112% at 10 mM | Lonicera japonica | |
Mn2+ | stimulates activity to 120% at 10 mM | Lonicera japonica | |
additional information | Na+, K+, Cl- and SO42- have no effect on enzyme activity | Lonicera japonica | |
Zn2+ | 122% activity at 0.1 mM | Lonicera japonica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
1 * 49000, SDS-PAGE | Lonicera japonica |
49277 | - |
1 * 49277, LC-ESI mass spectrometry | Lonicera japonica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lonicera japonica | - |
variety Yate | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and DEAE-cellulose column chromatography | Lonicera japonica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower bud | - |
Lonicera japonica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.145 | - |
using L-DOPA as substrate, crude enzyme, at pH 7.5 and 25°C | Lonicera japonica |
4.458 | - |
using L-DOPA as substrate, after 30.76fold purification, at pH 7.5 and 25°C | Lonicera japonica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | best substrate | Lonicera japonica | ? | - |
? | |
chlorogenic acid + O2 | - |
Lonicera japonica | ? | - |
? | |
L-DOPA + O2 | - |
Lonicera japonica | ? | - |
? | |
additional information | polyphenol oxidase shows no activity with L-tyrosine, digallol, gallic acid, and methyl gallate | Lonicera japonica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 49000, SDS-PAGE | Lonicera japonica |
monomer | 1 * 49277, LC-ESI mass spectrometry | Lonicera japonica |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Lonicera japonica |
PPO | - |
Lonicera japonica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Lonicera japonica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the residual soluble enzyme activity decreases significantly when temperature reaches 40°C | Lonicera japonica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Lonicera japonica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | the enzyme is stable within pH 8.0-10.0, retaining more than 80% of its activity. The enzyme is almost unstable and inactive when pH is below 3.0, only remaining less than 20% of its activity | Lonicera japonica |