Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Malus domestica | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a copper enzyme | Malus domestica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 4-methylcatechol + O2 | Malus domestica | - |
2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
2 catechol + O2 | Malus domestica | - |
2 1,2-benzoquinone + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
cv. Red Fuji | - |
Purification (Comment) | Organism |
---|---|
native enzyme 54.41fold by temperature-induced phase partitioning technique, dialysis, and ion exchange chromatography | Malus domestica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Malus domestica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
370004 U/mg for the purified native enzyme. One unit of enzyme activity is defined as a 0.001-unit change in absorbance per min per ml | Malus domestica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 4-methylcatechol + O2 | - |
Malus domestica | 2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
2 4-methylcatechol + O2 | best substrate | Malus domestica | 2 4-methyl-1,2-benzoquinone + 2 H2O | - |
? | |
2 catechol + O2 | - |
Malus domestica | 2 1,2-benzoquinone + 2 H2O | - |
? | |
additional information | the putative substrate-binding pocket contains six polar or charged amino acids, His191, His221, Trp224, Trp228, Phe227, and Val190. Trp224 and Trp228 form hydrogen bonds with 4-methylcatechol. Molecular docking of optimum substrate of mPPO, 4-methylcatechol, overview | Malus domestica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67300, SDS-PAGE | Malus domestica |
More | the three-dimensional structure of mPPO consists of six alpha-helices, two short beta-strands, and ten random coils, three-dimensional structure modeling based on the Vitis vinifera PPO, PDB ID 2P3X, as template | Malus domestica |
Synonyms | Comment | Organism |
---|---|---|
MppO | - |
Malus domestica |
polyphenol oxidase | - |
Malus domestica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Malus domestica |
General Information | Comment | Organism |
---|---|---|
additional information | three-dimensional structure modeling based on the Vitis vinifera PPO, PDB ID 2P3X, as template | Malus domestica |