Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDT-20 | the addition of 0.025% (v/v) EDT-20 in the assay increases the activity 3-4fold | Glycine max | |
EDT-20 | the addition of 0.025% (v/v) EDT-20 in the assay increases the activity 3-4fold | Arum italicum | |
pyruvate | potent activator, its presence is essential for maximum activity, pyruvate increases the activity of AOX but does not increase its affinity for its substrate | Glycine max | |
pyruvate | potent activator, its presence is essential for maximum activity, pyruvate increases the activity of AOX but does not increase its affinity for its substrate | Arum italicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
n-octyl gallate | the enzyme is fully sensitive to 0.001 mM octyl gallate | Arum italicum | |
n-octyl gallate | the enzyme is fully sensitive to 0.001 mM octyl gallate | Glycine max | |
n-propyl gallate | - |
Arum italicum | |
n-propyl gallate | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1153 | - |
ubiquinol-1 | in the presence of EDT-20 (0.025%) and pyruvate, 0.3 M succinate, 25 mM TES, 10 mM potassium phosphate, and 2 mM magnesium sulfate, pH 7.0, at 25°C | Glycine max | |
0.331 | - |
ubiquinol-1 | in the presence of EDT-20 (0.025%) and pyruvate, 0.3 M succinate, 25 mM TES, 10 mM potassium phosphate, and 2 mM magnesium sulfate, pH 7.0, at 25°C | Arum italicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | inner mitochondrial membrane | Glycine max | 31966 | - |
mitochondrial membrane | inner mitochondrial membrane | Arum italicum | 31966 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arum italicum | - |
- |
- |
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography and Q-Sepharose column chromatography | Arum italicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Glycine max | - |
spadix | - |
Arum italicum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
decylubiquinol + O2 | - |
Glycine max | decylubiquinone + H2O | - |
? | |
decylubiquinol + O2 | - |
Arum italicum | decylubiquinone + H2O | - |
? | |
duroquinol + O2 | - |
Glycine max | duroquinone + H2O | - |
? | |
duroquinol + O2 | - |
Arum italicum | duroquinone + H2O | - |
? | |
ubiquinol-1 + O2 | most suitable substrate | Glycine max | ubiquinone-1 + H2O | - |
? | |
ubiquinol-1 + O2 | most suitable substrate | Arum italicum | ubiquinone-1 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alternative oxidase | - |
Glycine max |
alternative oxidase | - |
Arum italicum |
AOX | - |
Glycine max |
AOX | - |
Arum italicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Glycine max |
7 | 7.5 | - |
Arum italicum |