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Literature summary for 1.10.3.2 extracted from

  • Xiao, Y.Z.; Tu, X.M.; Wang, J.; Zhang, M.; Cheng, Q.; Zeng, W.Y.; Shi, Y.Y.
    Purification, molecular characterization and reactivity with aromatic compounds of a laccase from basidiomycete Trametes sp. strain AH28-2 (2003), Appl. Microbiol. Biotechnol., 60, 700-707.
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
cyanide 0.1 mM, strong Trametes sp.
Sodium azide 0.1 mM, strong Trametes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 25°C Trametes sp.
0.0255
-
 2,6-dimethoxyphenol pH 4.5, 25°C Trametes sp.
0.42
-
 guaiacol pH 4.5, 25°C Trametes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 gel filtration Trametes sp.
58500
-
 1 * 62000, SDS-PAGE, 1 * 58500, MALDI mass spectrometry Trametes sp.
62000
-
 1 * 62000, SDS-PAGE, 1 * 58500, MALDI mass spectrometry Trametes sp.

Organism

Organism UniProt Comment Textmining
Trametes sp. Q5I7J0 two isozymes after induction by kraft lignin, laccase as forms 85% of total activity
-
Trametes sp. AH28-2 Q5I7J0 two isozymes after induction by kraft lignin, laccase as forms 85% of total activity
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content 11-12% Trametes sp.

Purification (Commentary)

Purification (Comment) Organism
isozyme laccase Trametes sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Trametes sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
66.8
-
 pH 4.5, 25°C Trametes sp.
79
-
 pH 4.5, 25°C Trametes sp.
670
-
 pH 4.5, 25°C Trametes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes sp. ? + H2O
-
 ?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
2,6-dichlorophenol + O2
-
 Trametes sp. ? + H2O
-
 ?
2,6-dichlorophenol + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
2,6-dimethoxyphenol + O2
-
 Trametes sp. ? + H2O
-
 ?
2,6-dimethoxyphenol + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
fluorene + O2
-
 Trametes sp. ? + H2O
-
 ?
fluorene + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
guaiacol + O2
-
 Trametes sp. ? + H2O
-
 ?
guaiacol + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 62000, SDS-PAGE, 1 * 58500, MALDI mass spectrometry Trametes sp.

Synonyms

Synonyms Comment Organism
laccase A
-
 Trametes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 substrate guaiacol Trametes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 27 min, 50% residual activity Trametes sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
69
-
 guaiacol pH 4.5, 25°C Trametes sp.
81
-
 2,6-dimethoxyphenol pH 4.5, 25°C Trametes sp.
692
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5, 25°C Trametes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 substrate guaiacol Trametes sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.2 8 good stability Trametes sp.

pI Value

Organism Comment pI Value Maximum pI Value
Trametes sp. isoelectric focusing
-
 4.2