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Literature summary for 1.10.3.2 extracted from

  • Kiiskinen, L.L.; Kruus, K.; Bailey, M.; Ylosmaki, E.; Siika-Aho, M.; Saloheimo, M.
    Expression of Melanocarpus albomyces laccase in Trichoderma reesei and characterization of the purified enzyme (2004), Microbiology, 150, 3065-3074.
    View publication on PubMed

Application

Application Comment Organism
biotechnology expression of non-fused enzyme and hydrophobin-enzyme fusion protein in Trichoderma reesei, intracellular accumulation and degradation of fusion protein, production of non-fused enzyme at up to 920 mg per l of fed-batch culture, purification from culture supernatant Melanocarpus albomyces

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
syringaldazine pH 6.0 Melanocarpus albomyces
0.0052
-
2,6-dimethoxyphenol pH 6.0 Melanocarpus albomyces
0.27
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5 Melanocarpus albomyces
0.91
-
guaiacol pH 6.0 Melanocarpus albomyces

Organism

Organism UniProt Comment Textmining
Melanocarpus albomyces
-
expression inTrichoderma reesei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
150
-
substrate guaiacol, pH 6.0 Melanocarpus albomyces
400
-
substrate 2,6-dimethoxypenol, pH 6.0 Melanocarpus albomyces
850
-
substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), pH 4.5 Melanocarpus albomyces
1070
-
substrate syringaldazine, pH 6.0 Melanocarpus albomyces

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Melanocarpus albomyces ? + H2O
-
?
2,6-dimethoxyphenol + O2
-
Melanocarpus albomyces ? + H2O
-
?
guaiacol + O2
-
Melanocarpus albomyces ? + H2O
-
?
syringaldazine + O2
-
Melanocarpus albomyces ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
31.7
-
guaiacol pH 6.0 Melanocarpus albomyces
67
-
2,6-dimethoxyphenol pH 6.0 Melanocarpus albomyces
78.3
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5 Melanocarpus albomyces
92
-
syringaldazine pH 6.0 Melanocarpus albomyces