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Literature summary for 1.10.3.2 extracted from

  • Xiao, Y.Z.; Chen, Q.; Hang, J.; Shi, Y.Y.; Wu, J.; Hong, Y.Z.; Wang, Y.P.
    Selective induction, purification and characterization of a laccase isozyme from the basidiomycete Trametes sp. AH28-2 (2004), Mycologia, 96, 26-35.
    View publication on PubMed
Search for more literature for 1.10.3.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
 syringaldazine pH 4.5 Trametes sp.
0.11
-
 2,6-dimethoxyphenol pH 4.5 Trametes sp.
0.177
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5 Trametes sp.
1.249
-
 guaiacol pH 4.5 Trametes sp.

Metals/Ions

Metals/Ions Comment Organism Structure
copper four ions per molecule Trametes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71450
-
 1 * 74000, SDS-PAGE, 1 * 71450, mass spectrometry Trametes sp.
72000
-
 gel filtration Trametes sp.
74000
-
 1 * 74000, SDS-PAGE, 1 * 71450, mass spectrometry Trametes sp.

Organism

Organism UniProt Comment Textmining
Trametes sp.
-
 induction in cellobiose-based culture medium, selective induction of isozyme B by 3,5-dihydroxytoluene
-
Trametes sp. AH28-2
-
 induction in cellobiose-based culture medium, selective induction of isozyme B by 3,5-dihydroxytoluene
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein about 25% carbohydrate Trametes sp.

Purification (Commentary)

Purification (Comment) Organism
-
 Trametes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes sp. ? + H2O
-
 ?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
2,6-dimethoxyphenol + O2
-
 Trametes sp. ? + H2O
-
 ?
2,6-dimethoxyphenol + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
guaiacol + O2
-
 Trametes sp. ? + H2O
-
 ?
guaiacol + O2
-
 Trametes sp. AH28-2 ? + H2O
-
 ?
syringaldazine + O2
-
 Trametes sp. ?
-
 ?
syringaldazine + O2
-
 Trametes sp. AH28-2 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 74000, SDS-PAGE, 1 * 71450, mass spectrometry Trametes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 substrate guaiacol Trametes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 14 min, 50% residual activity Trametes sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
140
-
 guaiacol pH 4.5 Trametes sp.
189
-
 2,6-dimethoxyphenol pH 4.5 Trametes sp.
600
-
 syringaldazine pH 4.5 Trametes sp.
2277
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 4.5 Trametes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
 substrate guaiacol Trametes sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 7.5
-
 Trametes sp.

pI Value

Organism Comment pI Value Maximum pI Value
Trametes sp. isoelectric focusing
-
 4