Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | stimulates, induction | Fomes sclerodermeus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | both isozymes show Michaelis-Menten kinetics, overview | Fomes sclerodermeus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 67000, both isozymes, SDS-PAGE | Fomes sclerodermeus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fomes sclerodermeus | - |
two isozymes Lac I and Lac II | - |
Fomes sclerodermeus BAFC 2752 | - |
two isozymes Lac I and Lac II | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 3% N-linked carbohydrate content for both isozymes | Fomes sclerodermeus |
Purification (Comment) | Organism |
---|---|
native isozymes Lac I and II by anion exchange chromatography and gel filtration, overview | Fomes sclerodermeus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enzyme production in presence or absence of exogenous copper, overview | Fomes sclerodermeus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Fomes sclerodermeus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Fomes sclerodermeus | ? | - |
? | |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Fomes sclerodermeus BAFC 2752 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | preferred substrate | Fomes sclerodermeus | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | i.e. coerulignone | ? | |
2,6-dimethoxyphenol + O2 | preferred substrate | Fomes sclerodermeus BAFC 2752 | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | i.e. coerulignone | ? | |
ferulic acid + O2 | - |
Fomes sclerodermeus | ? | - |
? | |
ferulic acid + O2 | - |
Fomes sclerodermeus BAFC 2752 | ? | - |
? | |
vanillic acid + O2 | - |
Fomes sclerodermeus | ? | - |
? | |
vanillic acid + O2 | - |
Fomes sclerodermeus BAFC 2752 | ? | - |
? | |
veratric acid + O2 | - |
Fomes sclerodermeus | ? | - |
? | |
veratric acid + O2 | - |
Fomes sclerodermeus BAFC 2752 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, both isozymes, SDS-PAGE | Fomes sclerodermeus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
182 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.0, isozyme Lac II | Fomes sclerodermeus | |
199 | - |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.0, isozyme Lac I | Fomes sclerodermeus | |
206 | - |
2,6-dimethoxyphenol | pH 5.0, isozyme Lac I | Fomes sclerodermeus | |
218 | - |
2,6-dimethoxyphenol | pH 5.0, isozyme Lac II | Fomes sclerodermeus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Fomes sclerodermeus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Fomes sclerodermeus | Lac I, isoelectric focusing | - |
3.41 |
Fomes sclerodermeus | Lac II, isoelectric focusing | - |
3.48 |