Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetic acid | - |
Phoma sp. | |
acetone | 31% (v/v) 50% inhibition | Phoma sp. | |
acetonitrile | 4.5% (v/v) 50% inhibition | Phoma sp. | |
DMSO | 40% (v/v) 50% inhibition | Phoma sp. | |
EDTA | - |
Phoma sp. | |
ethanol | 52% (v/v) 50% inhibition | Phoma sp. | |
formic acid | - |
Phoma sp. | |
Isopropanol | 60% (v/v) 50% inhibition | Phoma sp. | |
methanol | 52% (v/v) 50% inhibition | Phoma sp. | |
Na2CO3 | - |
Phoma sp. | |
NaCl | - |
Phoma sp. | |
NaF | - |
Phoma sp. | |
NaN3 | - |
Phoma sp. | |
Urea | - |
Phoma sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Phoma sp. | |
0.008 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 2.5, 65°C | Phoma sp. | |
0.02 | - |
syringaldazine | pH 5.0, 65°C | Phoma sp. | |
0.266 | - |
2,6-dimethoxyphenol | pH 4.0, 65°C | Phoma sp. | |
0.284 | - |
anthraquinonic dye Acid Blue 62 | pH 5.5, 65°C | Phoma sp. | |
0.648 | - |
guaiacol | pH 5.5, 65°C | Phoma sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Phoma sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | a blue copper-containing oxidoreductase | Phoma sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | 151200 | about, gel filtration and mass spectrometry | Phoma sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Phoma sp. | laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties | ? | - |
? | |
additional information | Phoma sp. UHH 5-1-03 / DSM 22425 | laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phoma sp. | B3TLA6 | isolated from Saale river Germany | - |
Phoma sp. UHH 5-1-03 / DSM 22425 | B3TLA6 | isolated from Saale river Germany | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylation site prediction reveals five putative sites, and nine putative sites for O-glycosylation, overview | Phoma sp. |
Purification (Comment) | Organism |
---|---|
native enzyme 117fold by hydrophobic interaction chromatography and gel filtration | Phoma sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.1 | - |
purified enzyme | Phoma sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Phoma sp. | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Phoma sp. UHH 5-1-03 / DSM 22425 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Phoma sp. | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Phoma sp. UHH 5-1-03 / DSM 22425 | ? | - |
? | |
anthraquinonic dye Acid Blue 62 + O2 | - |
Phoma sp. | ? | - |
? | |
guaiacol + O2 | - |
Phoma sp. | ? | - |
? | |
guaiacol + O2 | - |
Phoma sp. UHH 5-1-03 / DSM 22425 | ? | - |
? | |
additional information | laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties | Phoma sp. | ? | - |
? | |
additional information | laccases are able to oxidize a variety of substrates, ranging from mono- and polyphenols over aminophenols, methoxyphenols and aromatic amines to polycyclic aromatic hydrocarbons and certain lignin moieties | Phoma sp. UHH 5-1-03 / DSM 22425 | ? | - |
? | |
syringaldazine + O2 | - |
Phoma sp. | ? | - |
? | |
syringaldazine + O2 | - |
Phoma sp. UHH 5-1-03 / DSM 22425 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 74000-75600, about, mass spectrometry and SDS-PAGE, laccase undergoes pH-dependent dimerisation | Phoma sp. |
More | the enzyme contains a signal sequence and a C-terminal conserved DSGL motif, tryptic peptide analysis, overview | Phoma sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Phoma sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 45 | the enzyme is most stable between 4°C and room temperature. After 288 h, the recovered activities correspond to approximately 51% at 4°C, 57% at 15°C and 49% at room temperature of the initial activity. The laccase is nearly completely inactivated at 35°C after 288 h and at 45°C after 24 h | Phoma sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
syringaldazine | pH 5.0, 65°C | Phoma sp. | |
4.3 | - |
2,6-dimethoxyphenol | pH 4.0, 65°C | Phoma sp. | |
4.6 | - |
guaiacol | pH 5.5, 65°C | Phoma sp. | |
10.6 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 2.5, 65°C | Phoma sp. | |
32.2 | - |
anthraquinonic dye Acid Blue 62 | pH 5.5, 65°C | Phoma sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.5 | 5.5 | the optimal pH values for the different substrates 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid, 2,6-dimethoxyphenol, guaiacol, syringaldazine, and anthraquinonic dye Acid Blue 62 oxidation are pH 2.5, pH 4.5, pH 5.0, pH 5.5 and pH 5.5, respectively | Phoma sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | activity range, at pH 7.0, about 17%, 24%, 33%, 23% and 21% of the maximum oxidation rate is observed for substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid, 2,6-dimethoxyphenol, guaiacol, syringaldazine, and anthraquinonic dye Acid Blue 62, respectively, while approximately 2%, 8%, 15%, 4% and 17% of the maximum activity in oxidation of these substrates is obtained at pH 8.0 | Phoma sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | the laccase is most stable at pH 4.0, pH 7.0, and 8.0, retaining about 57%, 62%, and 65% of the initial activity, respectively, after 355 h. The enzyme is less stable at pH 5.0 and pH 6.0 and is completely inactivated within 355 h at pH 2.0 | Phoma sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Phoma sp. | isoelectric focussing | - |
8.3 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.11 | - |
pH 4.0, 65°C | Phoma sp. | NaN3 | |
0.23 | - |
pH 4.0, 65°C | Phoma sp. | formic acid | |
3.7 | - |
pH 4.0, 65°C | Phoma sp. | NaF | |
19 | - |
pH 4.0, 65°C | Phoma sp. | EDTA | |
69 | - |
pH 4.0, 65°C | Phoma sp. | Na2CO3 | |
80 | - |
pH 4.0, 65°C | Phoma sp. | NaCl | |
2250 | - |
pH 4.0, 65°C | Phoma sp. | Urea |