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Literature summary for 1.10.3.2 extracted from

  • Stepanova, E.V.; Fedorova, T.V.; Sorokina, O.N.; Volkov, V.V.; Koroleva, O.V.; Dembo, A.T.
    Effect of solvent phase transitions on enzymatic activity and structure of laccase from Coriolus hirsutus (2009), Biochemistry (Moscow), 74, 385-392.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information analysis of enzyme dynamics and catalytic, spectral properties. Analysis of the structural state of the purified native laccase in solution at the phase transition before freezing, at freezing, and subsequent thawing of enzyme solution, processes in the active site on environmental temperature changes, overview Trametes hirsuta

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Trametes hirsuta
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the structure of laccase active sites including all copper centers of types T1, T2, and T3 changes during the phase transition, overview Trametes hirsuta

Organism

Organism UniProt Comment Textmining
Trametes hirsuta
-
strain 072
-
Trametes hirsuta 72
-
strain 072
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme by a three-stage chromatographic purification to homogeneity Trametes hirsuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
syringaldazine + O2
-
Trametes hirsuta ?
-
?
syringaldazine + O2
-
Trametes hirsuta 72 ?
-
?

Subunits

Subunits Comment Organism
monomer laccase exists in solution as a mixture of monomeric and aggregated particles in the percent ratio 85:15, the ratio does not change on phase transitions, small-angle X-ray scattering, overview Trametes hirsuta
More the structure of laccase active sites including all copper centers of types T1, T2, and T3 changes during the phase transition, overview Trametes hirsuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
assay at Trametes hirsuta