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Literature summary for 1.10.3.2 extracted from
- Fermentation optimization and characterization of the laccase from Pleurotus ostreatus strain 10969 (2009), Enzyme Microb. Technol., 44, 426-433.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| industry | the laccase has a wide application in industrial processes, particularly in renewable bio-energy industry | Pleurotus ostreatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Pleurotus ostreatus |  |
| Ag+ | - |
Pleurotus ostreatus | |
| Ba2+ | - |
Pleurotus ostreatus | |
| Ca2+ | - |
Pleurotus ostreatus | |
| Co2+ | - |
Pleurotus ostreatus | |
| Cu2+ | - |
Pleurotus ostreatus | |
| DTT | - |
Pleurotus ostreatus | |
| EDTA | - |
Pleurotus ostreatus | |
| EGTA | - |
Pleurotus ostreatus | |
| Fe3+ | - |
Pleurotus ostreatus | |
| K+ | - |
Pleurotus ostreatus | |
| Mg2+ | - |
Pleurotus ostreatus | |
| Mn2+ | - |
Pleurotus ostreatus | |
| Na+ | - |
Pleurotus ostreatus | |
| NaN3 | - |
Pleurotus ostreatus | |
| Ni2+ | - |
Pleurotus ostreatus | |
| SDS | - |
Pleurotus ostreatus | |
| Zn2+ | - |
Pleurotus ostreatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pleurotus ostreatus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Pleurotus ostreatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pleurotus ostreatus | - |
- |
- |
| Pleurotus ostreatus 10969 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 3.67fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration to homogeneity | Pleurotus ostreatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | optimization of culture conditions for high yield of extracellular enzyme requiring Mg2+ and glucose, overview | Pleurotus ostreatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1077 | - |
purified enzyme | Pleurotus ostreatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | best substrate | Pleurotus ostreatus | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | best substrate | Pleurotus ostreatus 10969 | ? | - |
? | |
| 4-methyl catechol + O2 | - |
Pleurotus ostreatus | ? | - |
? | |
| 4-methyl catechol + O2 | - |
Pleurotus ostreatus 10969 | ? | - |
? | |
| catechol + O2 | low activity | Pleurotus ostreatus | ? | - |
? | |
| catechol + O2 | low activity | Pleurotus ostreatus 10969 | ? | - |
? | |
| hydroquinone + O2 | - |
Pleurotus ostreatus | ? | - |
? | |
| hydroquinone + O2 | - |
Pleurotus ostreatus 10969 | ? | - |
? | |
| additional information | no activity with ferulic acid and veratryl alcohol | Pleurotus ostreatus | ? | - |
? | |
| additional information | no activity with ferulic acid and veratryl alcohol | Pleurotus ostreatus 10969 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Pleurotus ostreatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
sharp drop in activity above | Pleurotus ostreatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | activity range | Pleurotus ostreatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified enzyme, 10 min, loss of 90% activity | Pleurotus ostreatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Pleurotus ostreatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 5 | 40% of maximal activity at below pH 2.5 and above pH 5.0 | Pleurotus ostreatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 2 | purified enzyme, 10 min, loss of 90% activity | Pleurotus ostreatus |
| 7 | - |
purified enzyme, 10 min, loss of 90% activity | Pleurotus ostreatus |
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