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Literature summary for 1.10.3.2 extracted from
- Laccase isoforms with unusual properties from the basidiomycete Steccherinum ochraceum strain 1833 (2008), J. Appl. Microbiol., 105, 2065-2075.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | high activity | Steccherinum ochraceum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | a copper enzyme with type I Cu2+ | Steccherinum ochraceum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
x * 57000, isozyme laccase I, SDS-PAGE, x * 59000, isozyme laccase II, SDS-PAGE, x * 63000, isozyme laccase III, SDS-PAGE | Steccherinum ochraceum |
| 59000 | - |
x * 57000, isozyme laccase I, SDS-PAGE, x * 59000, isozyme laccase II, SDS-PAGE, x * 63000, isozyme laccase III, SDS-PAGE | Steccherinum ochraceum |
| 63000 | - |
x * 57000, isozyme laccase I, SDS-PAGE, x * 59000, isozyme laccase II, SDS-PAGE, x * 63000, isozyme laccase III, SDS-PAGE | Steccherinum ochraceum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Steccherinum ochraceum | - |
three laccase isoforms: laccases I, II and III | - |
| Steccherinum ochraceum 1833 | - |
three laccase isoforms: laccases I, II and III | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native isozymes about 50fold by anion exchange chromatography and gel filtration | Steccherinum ochraceum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19 | - |
purified isozyme laccase I | Steccherinum ochraceum |
| 20.5 | - |
purified isozyme laccase III | Steccherinum ochraceum |
| 22.3 | - |
purified isozyme laccase II | Steccherinum ochraceum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Steccherinum ochraceum | ? | - |
? | |
| 2,4-dimethylphenol + O2 | - |
Steccherinum ochraceum | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Steccherinum ochraceum | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Steccherinum ochraceum 1833 | ? | - |
? | |
| 2,6-dimethylphenol + O2 | - |
Steccherinum ochraceum | ? | - |
? | |
| 2-methylphenol + O2 | i.e. guaiacol | Steccherinum ochraceum | ? | - |
? | |
| catechol + O2 | - |
Steccherinum ochraceum | ? | - |
? | |
| catechol + O2 | - |
Steccherinum ochraceum 1833 | ? | - |
? | |
| coniferyl alcohol + O2 | - |
Steccherinum ochraceum | ? | - |
? | |
| coniferyl alcohol + O2 | - |
Steccherinum ochraceum 1833 | ? | - |
? | |
| additional information | isozyme laccase II shows a higher activity and wider substrate specificity compared to the other isozymes, overview | Steccherinum ochraceum | ? | - |
? | |
| additional information | isozyme laccase II shows a higher activity and wider substrate specificity compared to the other isozymes, overview | Steccherinum ochraceum 1833 | ? | - |
? | |
| syringaldazine + O2 | - |
Steccherinum ochraceum | ? | - |
? | |
| syringaldazine + O2 | - |
Steccherinum ochraceum 1833 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57000, isozyme laccase I, SDS-PAGE, x * 59000, isozyme laccase II, SDS-PAGE, x * 63000, isozyme laccase III, SDS-PAGE | Steccherinum ochraceum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme is a polyphenol oxidase belonging to the family of multi-copper oxidases | Steccherinum ochraceum |
| p-diphenol dioxygen oxidoreductase | - |
Steccherinum ochraceum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
isozyme laccase I | Steccherinum ochraceum |
| 75 | - |
isozyme laccase II | Steccherinum ochraceum |
| 80 | - |
isozyme laccase III | Steccherinum ochraceum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 80 | - |
Steccherinum ochraceum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified isozymes, half-lives are 5-6 h, after 8 h 20% remaining activity for isozyme laccase I, 40% for laccase II and III | Steccherinum ochraceum |
| 70 | - |
purified isozymes, 8-15% remainng activity after 4 h, 50% remaining activity after 1.7 h | Steccherinum ochraceum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the pH optima of the isozymes with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid are in the range of pH 1.2-2.0 | Steccherinum ochraceum |
| 4 | 5 | isozyme laccase I, dependent on the substrate | Steccherinum ochraceum |
| 4.5 | 5 | isozyme laccase II | Steccherinum ochraceum |
| 4.5 | 5 | isozyme laccase III | Steccherinum ochraceum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 6 | - |
Steccherinum ochraceum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 6 | purified isozymes, 25°C, 1 week, stable | Steccherinum ochraceum |
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Release 2023.1 (January 2023)
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