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Literature summary for 1.10.3.2 extracted from
- Purification and characterization of an extracellular laccase from the anthracene-degrading fungus Fusarium solani MAS2 (2010), Biores. Technol., 101, 9772-9777.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| SDS | 131.1% relative activity in the presence of 2 mM SDS | Fusarium solani |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 20 mM | Fusarium solani | |
| Cd2+ | 71.1% residual activity at 20 mM | Fusarium solani | |
| Co2+ | 61.9% residual activity at 20 mM | Fusarium solani | |
| Cr3+ | 46.2% residual activity at 20 mM | Fusarium solani | |
| Cu2+ | 68.9% residual activity at 20 mM | Fusarium solani | |
| dithiothreitol | complete inhibition at 2 mM | Fusarium solani | |
| EDTA | 20.8% residual activity at 20 mM | Fusarium solani | |
| Fe2+ | 4.0% residual activity at 20 mM | Fusarium solani | |
| Fe3+ | complete inhibition at 20 mM | Fusarium solani | |
| Mn2+ | 49.1% residual activity at 20 mM | Fusarium solani | |
| NaN3 | complete inhibition at 2 mM | Fusarium solani | |
| Ni2+ | 62% residual activity at 20 mM | Fusarium solani | |
| Pb2+ | 97.2% residual activity at 20 mM | Fusarium solani | |
| SDS | 93.8% residual activity at 20 mM | Fusarium solani | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0086 | - |
2,6-dimethoxyphenol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
| 0.0794 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
| 2.657 | - |
guaiacol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
| 3.434 | - |
catechol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Fusarium solani | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | 130.7% relative activity in the presence of 20 mM Hg2+ | Fusarium solani | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
SDSPAGE | Fusarium solani |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | the laccase retains about 60% of its activity in the presence of 5% (v/v) acetone | Fusarium solani |
| acetonitrile | the laccase retains about 75% of its activity in the presence of 5% (v/v) acetonitrile | Fusarium solani |
| DMSO | the laccase retains about 70% of its activity in the presence of 5% (v/v) DMSO | Fusarium solani |
| Ethanol | the laccase retains about 75% of its activity in the presence of 5% (v/v) ethanol | Fusarium solani |
| Methanol | the laccase retains more than 75-80% of its activity in the presence of 5% (v/v) methanol | Fusarium solani |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium solani | - |
- |
- |
| Fusarium solani MAS2 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Fusarium solani |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap DEAE column chromatography and Sephacryl S-200 gel filtration | Fusarium solani |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.19 | - |
crude culture, at 30°C, pH 4.5 | Fusarium solani |
| 358.7 | - |
after 69.1fold purification, at 30°C, pH 4.5 | Fusarium solani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Fusarium solani | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Fusarium solani MAS2 | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | best substrate | Fusarium solani | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | best substrate | Fusarium solani MAS2 | ? | - |
? | |
| catechol + O2 | - |
Fusarium solani | ? | - |
? | |
| catechol + O2 | - |
Fusarium solani MAS2 | ? | - |
? | |
| guaiacol + O2 | - |
Fusarium solani | ? | - |
? | |
| guaiacol + O2 | - |
Fusarium solani MAS2 | ? | - |
? | |
| syringaldazine + O2 | - |
Fusarium solani | ? | - |
? | |
| syringaldazine + O2 | - |
Fusarium solani MAS2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 72000, native PAGE | Fusarium solani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| benzenediol: oxygen oxidoreductase | - |
Fusarium solani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Fusarium solani |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | the enzyme retains activity after incubation at 50°C for 1 h, but the activity sharply decreases when the temperature is increased to 60°C and almost no activity is detected at 70°C. The laccase can still oxidize 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) when incubated at 70°C for 5 min, but when the enzyme is incubated under the same condition without substrate, it loses almost all its activity | Fusarium solani |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28.4 | - |
guaiacol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
| 44.9 | - |
catechol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
| 107.1 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
| 121 | - |
2,6-dimethoxyphenol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
- |
Fusarium solani |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 12 | the purified laccase maintains stable at pH 3.0-11.0. The enzyme remains active at pH values from 3.0 to 12.0, and its activity at pH 2.0 and 12.0 are 56.6% and 85.5% of that at pH 7.0, respectively | Fusarium solani |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11 | - |
guaiacol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
| 13 | - |
catechol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
| 1300 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
| 14000 | - |
2,6-dimethoxyphenol | at 30°C in 100 mM sodium tartrate buffer (pH 4.5) | Fusarium solani | |
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