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Literature summary for 1.10.3.2 extracted from

  • Chen, S.C.; Wu, P.H.; Su, Y.C.; Wen, T.N.; Wei, Y.S.; Wang, N.C.; Hsu, C.A.; Wang, A.H.; Shyur, L.F.
    Biochemical characterization of a novel laccase from the basidiomycete fungus Cerrena sp. WR1 (2012), Protein Eng. Des. Sel., 25, 761-769.
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
gene lcc3, DNA and amino acid sequence determination and analysis, phylogenetic analysis Cerrena sp. WR1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 50°C Cerrena sp. WR1
0.393
-
 2,6-dimethoxyphenol pH 3.0, 50°C Cerrena sp. WR1
0.849
-
 guaiacol pH 3.0, 50°C Cerrena sp. WR1
0.881
-
 catechol pH 3.0, 50°C Cerrena sp. WR1

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Cerrena sp. WR1
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57500
-
 x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE Cerrena sp. WR1
64100
-
 x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE Cerrena sp. WR1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4 catechol + O2 Cerrena sp. WR1
-
 4 1,2-benzoquinone + 2 H2O
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitril 77.5% activity remaining after 14 days in 25% Cerrena sp. WR1
dimethyl sulfoxide 55.8% activity remaining after 14 days in 25% Cerrena sp. WR1
Ethanol 84.8% activity remaining after 14 days in 25% Cerrena sp. WR1
Methanol 74.6% activity remaining after 14 days in 25% Cerrena sp. WR1
N,N-dimethylformamide 81.2% activity remaining after 14 days in 25% Cerrena sp. WR1

Organism

Organism UniProt Comment Textmining
Cerrena sp. WR1 E7BLR0 gene lcc3
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein laccase Lcc3 is N-linked glycosylated, deglycosylation by peptide N-glycosidase F, the enzyme contains six potential N-glycosylation sites, i.e. N35, N236, N272, N311, N453 and N489, with a conserved sequence of NxS/T, ire found in the deduced Lcc3 amino acid sequence Cerrena sp. WR1

Purification (Commentary)

Purification (Comment) Organism
native enzyme 24.9fold from strain WR1, by two steps of anion exchange chromatography to over 96% homogeneity Cerrena sp. WR1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
144.3
-
 specific activity of the crude laccase broth, pH 3.0, 50°C Cerrena sp. WR1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 2,6-dimethoxyphenol + O2
-
 Cerrena sp. WR1 ?
-
 ?
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Cerrena sp. WR1 ?
-
 ?
4 catechol + O2
-
 Cerrena sp. WR1 4 1,2-benzoquinone + 2 H2O
-
 ?
4 guaiacol + O2
-
 Cerrena sp. WR1 ?
-
 ?
additional information the enzyme efficiently performs dye decoloration of anthraquinone dye RBBR Cerrena sp. WR1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 64100, glycosylated enzyme, SDS-PAGE, x * 57500, deglycosylated enzyme, SDS-PAGE Cerrena sp. WR1

Synonyms

Synonyms Comment Organism
Lcc3
-
 Cerrena sp. WR1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Cerrena sp. WR1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 Tm of Lcc3 is determined at 73.9°C and it shows a t1/2 of 120 min at 50°C Cerrena sp. WR1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
109.2
-
 2,6-dimethoxyphenol pH 3.0, 50°C Cerrena sp. WR1
115.5
-
 catechol pH 3.0, 50°C Cerrena sp. WR1
147.9
-
 guaiacol pH 3.0, 50°C Cerrena sp. WR1
934.6
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 50°C Cerrena sp. WR1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 with substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena sp. WR1
3.5
-
 with substrate 2,6-dimethoxyphenol Cerrena sp. WR1
4.5
-
 with substrate guaiacol Cerrena sp. WR1

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 loss of 50% activity after 43 h, 25°C Cerrena sp. WR1
4
-
 loss of 50% activity after 82 h, 25°C Cerrena sp. WR1
5
-
 loss of 20% activity after 100 h, 25°C Cerrena sp. WR1
6 10 no loss of activity after 100 h, 25°C Cerrena sp. WR1

General Information

General Information Comment Organism
evolution the enzyme belongs to the blue multicopper oxidase superfamily Cerrena sp. WR1