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Literature summary for 1.10.3.2 extracted from

  • Csarman, F.; Obermann, T.; Zanjko, M.C.; Man, P.; Halada, P.; Seiboth, B.; Ludwig, R.
    Functional expression and characterization of two laccases from the brown rot Fomitopsis pinicola (2021), Enzyme Microb. Technol., 148, 109801 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Activating Compound

Activating Compound Comment Organism Structure
acetic acid FpLcc2 is activated by acetic acid up to 3.7times. This activation effect is much more pronounced at pH 5.0 compared to pH 3.0 and can already be observed at a concentration of 1 mM Fomitopsis pinicola

Cloned(Commentary)

Cloned (Comment) Organism
heterologously produced using Trichoderma reesei QM9414 DELTAxyr1 as expression host Fomitopsis pinicola

Inhibitors

Inhibitors Comment Organism Structure
Citric acid
-
 Fomitopsis pinicola
oxalic acid
-
 Fomitopsis pinicola

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0351
-
 2,6-dimethoxyphenol pH 5.0, 30°C Fomitopsis pinicola
0.0509
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.0, 30°C Fomitopsis pinicola
0.0595
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C Fomitopsis pinicola
0.448
-
 2,6-dimethoxyphenol pH 3.0, 30°C Fomitopsis pinicola
0.891
-
 guaiacol pH 5.0, 30°C Fomitopsis pinicola
2.14
-
 guaiacol pH 3.0, 30°C Fomitopsis pinicola
8.01
-
 2,6-dimethoxyphenol pH 5.0, 30°C Fomitopsis pinicola
20.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C Fomitopsis pinicola
22.4
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.0, 30°C Fomitopsis pinicola
64.4
-
 2,6-dimethoxyphenol pH 3.0, 30°C Fomitopsis pinicola
918
-
 guaiacol pH 5.0, 30°C Fomitopsis pinicola
1790
-
 guaiacol pH 3.0, 30°C Fomitopsis pinicola

Organism

Organism UniProt Comment Textmining
Fomitopsis pinicola S8FGV1
-
-
Fomitopsis pinicola S8FIE4
-
-
Fomitopsis pinicola FP-58527 S8FGV1
-
-
Fomitopsis pinicola FP-58527 S8FIE4
-
-

Purification (Commentary)

Purification (Comment) Organism
purified to homogeneity by consecutive steps of hydrophobic interaction, anion exchange and size exclusion chromatography Fomitopsis pinicola

Storage Stability

Storage Stability Organism
highest recovery of enzymatic activity after freezing and thawing is observed by storing both laccase in 50 mM sodium phosphate buffer at pH 8.0 with the addition of 30 % (w/v) glycerol and 3.0 % sucrose Fomitopsis pinicola

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Fomitopsis pinicola ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Fomitopsis pinicola FP-58527 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Fomitopsis pinicola ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Fomitopsis pinicola FP-58527 ?
-
 ?
guaiacol + O2
-
 Fomitopsis pinicola 6-methoxycyclohexa-2,4-dienone + H2O
-
 ?
guaiacol + O2
-
 Fomitopsis pinicola FP-58527 6-methoxycyclohexa-2,4-dienone + H2O
-
 ?

Synonyms

Synonyms Comment Organism
FpLcc1
-
 Fomitopsis pinicola
FpLcc2
-
 Fomitopsis pinicola

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.89
-
 2,6-dimethoxyphenol pH 5.0, 30°C Fomitopsis pinicola
13.2
-
 guaiacol pH 5.0, 30°C Fomitopsis pinicola
14.8
-
 2,6-dimethoxyphenol pH 5.0, 30°C Fomitopsis pinicola
16.7
-
 guaiacol pH 5.0, 30°C Fomitopsis pinicola
23.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.0, 30°C Fomitopsis pinicola
42.6
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.0, 30°C Fomitopsis pinicola
48
-
 guaiacol pH 3.0, 30°C Fomitopsis pinicola
85.1
-
 guaiacol pH 3.0, 30°C Fomitopsis pinicola
102
-
 2,6-dimethoxyphenol pH 3.0, 30°C Fomitopsis pinicola
155
-
 2,6-dimethoxyphenol pH 3.0, 30°C Fomitopsis pinicola
315
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C Fomitopsis pinicola
409
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C Fomitopsis pinicola

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 pH-optima below 3, substrates: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 2,6-dimethoxyphenol Fomitopsis pinicola
3.5
-
 substrate: guaiacol Fomitopsis pinicola

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.181
-
 guaiacol pH 5.0, 30°C Fomitopsis pinicola
0.268
-
 guaiacol pH 3.0, 30°C Fomitopsis pinicola
14.8
-
 guaiacol pH 5.0, 30°C Fomitopsis pinicola
15.6
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C Fomitopsis pinicola
18.5
-
 2,6-dimethoxyphenol pH 5.0, 30°C Fomitopsis pinicola
19.03
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.0, 30°C Fomitopsis pinicola
24.02
-
 2,6-dimethoxyphenol pH 3.0, 30°C Fomitopsis pinicola
41.3
-
 guaiacol pH 3.0, 30°C Fomitopsis pinicola
225
-
 2,6-dimethoxyphenol pH 5.0, 30°C Fomitopsis pinicola
228
-
 2,6-dimethoxyphenol pH 3.0, 30°C Fomitopsis pinicola
455
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.0, 30°C Fomitopsis pinicola
6880
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 30°C Fomitopsis pinicola