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Literature summary for 1.10.3.3 extracted from

  • Stark, G.R.; Dawson, C.R.
    Ascorbic acid oxidase (1963), The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. ), 8, 297-311.
No PubMed abstract available
Search for more literature for 1.10.3.3

General Stability

General Stability Organism
gelatin, catalase, peroxidase and methemoglobin protect against inactivation Cucurbita pepo condensa

Inhibitors

Inhibitors Comment Organism Structure
3,4,5-hydroxybenzoic acid propyl ester
-
 Cucurbita pepo condensa
3,4-Dichlorophenylserine
-
 Cucurbita pepo condensa
8-hydroxyquinoline
-
 Cucurbita pepo condensa
alpha-tocopherol
-
 Cucurbita pepo condensa
Anthocyanin pigments
-
 Cucurbita pepo condensa
Auxin analogs
-
 Cucurbita pepo condensa
azide
-
 Cucurbita pepo condensa
Carotenes
-
 Cucurbita pepo condensa
Cu2+
-
 Cucurbita pepo condensa
Cupferron
-
 Cucurbita pepo condensa
cyanide
-
 Cucurbita pepo condensa
deoxycorticosterone
-
 Cucurbita pepo condensa
diethyldithiocarbamate
-
 Cucurbita pepo condensa
ethyl xanthate
-
 Cucurbita pepo condensa
H2O2
-
 Cucurbita pepo condensa
H2S
-
 Cucurbita pepo condensa
Hg2+ 0.01 mM, appreciable inhibition Cucurbita pepo condensa
iodoacetate
-
 Cucurbita pepo condensa
leucocyanidol
-
 Cucurbita pepo condensa
additional information natural inhibitors: cabbage extract, tomato extract, strawberry juice, extract of lemons, oranges, parsley, hips, leeks Cucurbita pepo condensa
Ni2+ some authors found inhibition, others not Cucurbita pepo condensa
nordihydroguaiaretic acid
-
 Cucurbita pepo condensa
organic mercurials
-
 Cucurbita pepo condensa
p-mercuribenzoate some authors report inhibition, others do not Cucurbita pepo condensa
Pyridine-KCNS
-
 Cucurbita pepo condensa
Salicylaldoxime
-
 Cucurbita pepo condensa
SO2
-
 Cucurbita pepo condensa
Tetraethylthiuramidisulfide
-
 Cucurbita pepo condensa
thiamine
-
 Cucurbita pepo condensa
Thiourea
-
 Cucurbita pepo condensa
Zn2+ some authors found inhibition, others not Cucurbita pepo condensa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 L-ascorbate spectrometric assay Cucurbita pepo condensa
0.24
-
 L-ascorbate spectrometric assay Cucurbita pepo condensa
0.42
-
 L-ascorbate manomeric assay following the rate of O2 uptake Cucurbita pepo condensa
2
-
 L-ascorbate manomeric assay following the rate of O2 uptake Cucurbita pepo condensa
5
-
 L-ascorbate manomeric assay following the rate of O2 uptake Cucurbita pepo condensa

Metals/Ions

Metals/Ions Comment Organism Structure
copper a multicopper protein, 6 atoms of copper per enzyme molecule Cucurbita pepo condensa

Organism

Organism UniProt Comment Textmining
Cucurbita pepo condensa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme may be a protein-copper carbohydrate complex Cucurbita pepo condensa

Reaction

Reaction Comment Organism Reaction ID
4 L-ascorbate + O2 = 4 monodehydroascorbate + 2 H2O mechanism: Cu2+ is reduced to Cu+, which is then reoxidized by oxygen Cucurbita pepo condensa
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 hydroquinone + O2 at pH 7.2 Cucurbita pepo condensa 2 quinone + 2 H2O
-
 ?
2 L-ascorbate + O2 no appreciable activity with: p-phenylenediamine, Na2S2O3, glutathione and cysteine Cucurbita pepo condensa 2 L-dehydroascorbate + 2 H2O
-
 ?
2,5-dichlorohydroquinone + O2
-
 Cucurbita pepo condensa 2,5-dichloroquinone + H2O
-
 ?
2,6-dichlorohydroquinone + O2
-
 Cucurbita pepo condensa 2,6-dichloroquinone + H2O
-
 ?
D-glucoascorbic acid + O2
-
 Cucurbita pepo condensa D-glucodehydroascorbate + H2O
-
 ?
D-isoascorbic acid + O2
-
 Cucurbita pepo condensa 2-dehydroisoascorbate + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4170
-
 L-ascorbate manometric assay Cucurbita pepo condensa
10800
-
 L-ascorbate spectrometric assay Cucurbita pepo condensa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Cucurbita pepo condensa

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 irreversible loss of activity below Cucurbita pepo condensa