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Literature summary for 1.10.3.3 extracted from
- Ascorbate oxidase (1979), Methods Enzymol., 62, 30-39.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | - |
Cucumis sativus |  |
| azide | - |
Cucurbita pepo condensa | |
| azide | competitive vs. ascorbate, noncompetitive vs. O2 | Cucurbita pepo medullosa | |
| F- | - |
Cucumis sativus | |
| F- | - |
Cucurbita pepo condensa | |
| F- | competitive vs. ascorbate, noncompetitive vs. O2 | Cucurbita pepo medullosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | - |
Cucurbita pepo condensa | |
| copper | - |
Cucurbita pepo medullosa | |
| copper | 10 copper atoms per enzyme molecule | Cucumis sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
2 * 70000, 70000 Da subunit consists of 2 polypeptide chains of 30000 and 40000 Da respectively | Cucurbita pepo medullosa |
| 140000 | - |
- |
Cucumis sativus |
| 140000 | - |
sedimentation equilibrium and difussion studies | Cucurbita pepo medullosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis sativus | - |
- |
- |
| Cucurbita pepo condensa | - |
- |
- |
| Cucurbita pepo medullosa | - |
green zucchini squash | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cucurbita pepo condensa |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reconstitution of copper-free apoascorbate oxidase with cuprous copper solution | Cucurbita pepo medullosa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Cucumis sativus | - |
| fruit | - |
Cucurbita pepo condensa | - |
| fruit | - |
Cucurbita pepo medullosa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-ascorbate + O2 | - |
Cucumis sativus | 2 L-dehydroascorbate + 2 H2O | - |
? | |
| 2 L-ascorbate + O2 | - |
Cucurbita pepo condensa | 2 L-dehydroascorbate + 2 H2O | - |
? | |
| 2 L-ascorbate + O2 | - |
Cucurbita pepo medullosa | 2 L-dehydroascorbate + 2 H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Cucumis sativus |
| dimer | 2 * 70000, 70000 Da subunit consists of 2 polypeptide chains of 30000 and 40000 Da respectively | Cucurbita pepo medullosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Cucurbita pepo medullosa |
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Release 2023.1 (January 2023)
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