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Literature summary for 1.10.3.3 extracted from
- Fabrication of an amperometric ascorbate biosensor using egg shell membrane bound Lagenaria siceraria fruit ascorbate oxidase (2010), J. Mol. Catal. B, 67, 66-71.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
L-ascorbate | free ascorbate oxidase, at pH 5.5 and 40°C | Lagenaria siceraria |  |
| 0.2 | - |
L-ascorbate | immobilized ascorbate oxidase, at pH 6.0 and 40°C | Lagenaria siceraria | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Lagenaria siceraria | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lagenaria siceraria | - |
bottle gourd | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sephacel column chromatography, and Sephadex G-100 gel filtration | Lagenaria siceraria |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Lagenaria siceraria | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, ascorbate oxidase immobilized onto egg shell membrane through glutaraldehyde coupling in 0.1 M sodium phosphate EDTA buffer pH 5.6, 120 days, 50% loss of activity | Lagenaria siceraria |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-ascorbate + O2 | - |
Lagenaria siceraria | 2 L-dehydroascorbate + 2 H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ascorbate oxidase | - |
Lagenaria siceraria |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
optimal pH of freee and biosensor/immobilized ascorbate oxidase | Lagenaria siceraria |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
optimal pH of free ascorbate oxidase | Lagenaria siceraria |
| 6 | - |
optimal pH of biosensor/immobilized ascorbate oxidase | Lagenaria siceraria |
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Release 2023.1 (January 2023)
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