Cloned (Comment) | Organism |
---|---|
the His-tagged enzyme is expressed in the Sf9 Spodoptera frugiperda ovarian cell line ATCC CRL-1711 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chrysoeriol | - |
Homo sapiens | |
Melatonin | QR2 is inhibited in the micromolar range by melatonin, but not when using N-benzyl dihydronicotinamide and coenzyme Q2 are used as substrates | Homo sapiens | |
additional information | dicoumarol is inactive up to 0.1 mM | Homo sapiens | |
resveratrol | - |
Homo sapiens | |
S26695 | i.e. N-[2-(7-methylaminosulfonyl-1-naphthyl)ethyl]acetamide | Homo sapiens | |
S29434 | i.e. N-[2-(2-methoxy-6H-dipyrido[2,3-a:3,2-e]pyrrolizin-11-yl)ethyl]-2-furamide, inhibits QR2 activity with an IC50 in the low nanomolar range from 0.7 to 80 nM, depending on the substrates and co-substrates used | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0102 | - |
2,6-dichlorophenolindophenol | using N-benzyl dihydronicotinamide as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | |
0.0111 | - |
menadione | using N-benzyl dihydronicotinamide as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | |
0.0113 | - |
N-benzyl dihydronicotinamide | using menadione as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | |
0.0137 | - |
coenzyme Q0 | using N-benzyl dihydronicotinamide as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | |
0.0361 | - |
N-benzyl dihydronicotinamide | using coenzyme Q0 as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | |
0.0387 | - |
coenzyme Q2 | using N-benzyl dihydronicotinamide as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | |
0.0404 | - |
N-benzyl dihydronicotinamide | using 2,6-dichlorophenolindophenol as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | |
0.0451 | - |
N-benzyl dihydronicotinamide | using coenzyme Q1 as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | |
0.0517 | - |
coenzyme Q1 | using N-benzyl dihydronicotinamide as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | |
0.0602 | - |
N-benzyl dihydronicotinamide | using coenzyme Q2 as cosubstrate, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme does not accept conventional phosphorylated nicotinamides as hydride donors | Homo sapiens | ? | - |
? | |
N-benzyldihydronicotinamide + 2,6-dichlorophenolindophenol | - |
Homo sapiens | N-benzylnicotinamide + reduced 2,6-dichlorophenolindophenol | - |
? | |
N-benzyldihydronicotinamide + menadione | - |
Homo sapiens | N-benzylnicotinamide + menadiol | - |
? | |
N-benzyldihydronicotinamide + oxidized coenzyme Q0 | - |
Homo sapiens | N-benzylnicotinamide + reduced coenzyme Q20 | - |
? | |
N-benzyldihydronicotinamide + oxidized coenzyme Q1 | - |
Homo sapiens | N-benzylnicointamide + reduced coenzyme Q1 | - |
? | |
N-benzyldihydronicotinamide + oxidized coenzyme Q2 | - |
Homo sapiens | N-benzylnicotinamide + reduced coenzyme Q2 | - |
? | |
nicotinamide riboside + menadiol | - |
Homo sapiens | dihydronicotinamide riboside + menadione | - |
? | |
nicotinamide riboside + reduced 2,6-dichlorophenolindophenol | - |
Homo sapiens | dihydronicotinamide riboside + 2,6-dichlorophenolindophenol | - |
? | |
nicotinamide riboside + reduced coenzyme Q0 | - |
Homo sapiens | dihydronicotinamide riboside + coenzyme Q0 | - |
? | |
nicotinamide riboside + reduced coenzyme Q1 | - |
Homo sapiens | dihydronicotinamide riboside + coenzyme Q1 | - |
? | |
nicotinamide riboside + reduced coenzyme Q2 | - |
Homo sapiens | dihydronicotinamide riboside + coenzyme Q2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
QR2 | - |
Homo sapiens |
quinone reductase 2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000007 | - |
using nicotinamide riboside and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | S29434 | |
0.000005 | - |
using nicotinamide riboside and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | S29434 | |
0.0000067 | - |
using N-benzyl dihydronicotinamide and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | S29434 | |
0.000016 | - |
using nicotinamide riboside and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | chrysoeriol | |
0.000076 | - |
using nicotinamide riboside and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | chrysoeriol | |
0.000079 | - |
using N-benzyl dihydronicotinamide and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | S29434 | |
0.000129 | - |
using nicotinamide riboside and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | resveratrol | |
0.000143 | - |
using N-benzyl dihydronicotinamide and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | resveratrol | |
0.00016 | - |
using N-benzyl dihydronicotinamide and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | chrysoeriol | |
0.00082 | - |
using N-benzyl dihydronicotinamide and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | chrysoeriol | |
0.0012 | - |
using nicotinamide riboside and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | S26695 | |
0.0025 | - |
using N-benzyl dihydronicotinamide and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | S26695 | |
0.0028 | - |
using nicotinamide riboside and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | Melatonin | |
0.0029 | - |
using nicotinamide riboside and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | resveratrol | |
0.0126 | - |
using nicotinamide riboside and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | S26695 | |
0.0151 | - |
using N-benzyl dihydronicotinamide and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | S26695 | |
0.0397 | - |
using nicotinamide riboside and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | Melatonin | |
0.0415 | - |
using N-benzyl dihydronicotinamide and menadione as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | Melatonin | |
0.07 | - |
using N-benzyl dihydronicotinamide and coenzyme Q2 as substrates, at 25°C, in 50 mM Tris-HCl, pH 8.5 | Homo sapiens | resveratrol |