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Literature summary for 1.11.1.11 extracted from

  • Shigeoka, S.; Nahana, Y.; Kitaoka, S.
    Purification and some properties of L-ascorbic-acid-specific peroxidase in Euglena gracilis Z (1980), Arch. Biochem. Biophys., 201, 121-127.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Al3+
-
Euglena gracilis
Br- marked inhibition at 1 mM Euglena gracilis
Ca2+
-
Euglena gracilis
EDTA slight inhibition, but when the enzyme is incubated with EDTA 1 mM at 37°C for 3 min in the absence of sucrose and ferrous sulfate there is nearly complete inhibition Euglena gracilis
F-
-
Euglena gracilis
Hg2+ complete inhibition at 1 mM Euglena gracilis
I-
-
Euglena gracilis
KCN 96.4% inhibition at 1 mM Euglena gracilis
Li+
-
Euglena gracilis
Mg2+
-
Euglena gracilis
Mn2+ marked inhibition at 1 mM Euglena gracilis
NaN3 91.5% inhibition at 1 mM Euglena gracilis
Ni2+
-
Euglena gracilis
Zn2+
-
Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
H2O2 substrate: L-ascorbic acid Euglena gracilis
0.22
-
H2O2 substrate: pyrogallol Euglena gracilis
0.41
-
L-ascorbic acid
-
Euglena gracilis
9.6
-
pyrogallol
-
Euglena gracilis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe hemoprotein Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ascorbate + H2O2 Euglena gracilis the enzyme works for protection of cell membrane, by reducing the peroxide compounds generated endogenously from unsaturated fatty acids dehydroascorbate + 2 H2O
-
?
L-ascorbate + H2O2 Euglena gracilis physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation dehydroascorbate + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ultracentrifugation, column chromatography on DEAE-cellulose, ammonium sulfate precipitation and column chromatography on Sephadex G-150, DEAE-cellulose and CM-cellulose Euglena gracilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8
-
purified enzyme, electron donor: glutathione, electron acceptor: H2O2 Euglena gracilis
5.8
-
purified enzyme, electron donor: iodide, electron acceptor: H2O2 Euglena gracilis
18
-
purified enzyme, electron donor: reductic acid, electron acceptor: H2O2 Euglena gracilis
20.3
-
purified enzyme, electron donor: guaiacol, electron acceptor: H2O2 Euglena gracilis
132.2
-
purified enzyme, electron donor: L-ascorbic acid, electron acceptor: cumene hydroperoxide Euglena gracilis
142.1
-
purified enzyme, electron donor: D-araboascorbic acid, electron acceptor: H2O2 Euglena gracilis
172.8
-
purified enzyme, electron donor: L-ascorbic acid, electron acceptor: tert-butyl hydroperoxide Euglena gracilis
185.5
-
purified enzyme, electron donor: pyrogallol, electron acceptor: H2O2 Euglena gracilis
254
-
purified enzyme, electron donor: L-ascorbic acid, electron acceptor: H2O2 Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytochrome c + H2O2 no activity Euglena gracilis ? + H2O
-
?
D-araboascorbic acid + H2O2 56% activity relative to L-ascorbate Euglena gracilis dehydroascorbate + H2O
-
?
guaiacol + H2O2 8% activity relative to L-ascorbate Euglena gracilis ?
-
?
iodide + H2O2 2.3% activity relative to L-ascorbate Euglena gracilis ?
-
?
L-ascorbate + H2O2 tert-butyl hydroperoxide and cumene hydroperoxide also serve as electron acceptor Euglena gracilis dehydroascorbate + H2O
-
?
L-ascorbate + H2O2 the enzyme works for protection of cell membrane, by reducing the peroxide compounds generated endogenously from unsaturated fatty acids Euglena gracilis dehydroascorbate + 2 H2O
-
?
L-ascorbate + H2O2 physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation Euglena gracilis dehydroascorbate + 2 H2O
-
?
L-ascorbic acid + cumene hydroperoxide
-
Euglena gracilis dehydroascorbate + 1,1-dimethylbenzylalcohol + H2O
-
?
L-ascorbic acid + tert-butylhydroperoxide
-
Euglena gracilis dehydroascorbate + tert-butylalcohol
-
?
additional information the activity with glutathione is less than 1.1% of that with L-ascorbic acid, no activity with: cytochrome c, NADH, NADPH, palmitic acid and triose reductone Euglena gracilis ?
-
?
NADPH + H2O2 no activity Euglena gracilis ? + H2O
-
?
pyrogallol + H2O2 73.1% activity relative to L-ascorbate Euglena gracilis 3-hydroxybenzo-1,2-quinone + H2O
-
?
reductic acid + H2O2 i.e. 2,3-dihydroxy-2-cyclopenten-1-one, 7.1% activity relative to L-ascorbate Euglena gracilis ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
assay at Euglena gracilis
32 34
-
Euglena gracilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
maximum activity maintained up to Euglena gracilis
40
-
inactivated after 5 min Euglena gracilis
40
-
preincubated in 30% sucrose and 0.05 mM ferrous sulfate, retains 88.1% activity after 5 min Euglena gracilis
52
-
complete inactivation Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Euglena gracilis

pH Range

pH Minimum pH Maximum Comment Organism
6 7.9 maximum activity maintained Euglena gracilis

Cofactor

Cofactor Comment Organism Structure
heme
-
Euglena gracilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0018
-
KCN competitive inhibition against H2O2 Euglena gracilis
0.0083
-
KCN uncompetitive inhibition against L-ascorbic acid Euglena gracilis
0.059
-
NaN3 uncompetitive inhibition against H2O2 Euglena gracilis
0.085
-
NaN3 competitive inhibition against L-ascorbic acid Euglena gracilis