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Literature summary for 1.11.1.11 extracted from

  • Dalton, D.A.; Diaz del Castillo, L.; Kahn, M.L.; Joyner, S.L.; Chatfield, J.M.
    Heterologous expression and characterization of soybean cytosolic ascorbate peroxidase (1996), Arch. Biochem. Biophys., 328, 1-8.
    View publication on PubMed
Search for more literature for 1.11.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, most of the enzyme produced is present in the apo-form, without heme Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 H2O2 recombinant enzyme Glycine max
0.35
-
 ascorbate recombinant enzyme Glycine max
1.8
-
 guaiacol recombinant enzyme Glycine max
2.4
-
 pyrogallol recombinant enzyme Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Glycine max 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-ascorbate + H2O2 Glycine max physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation dehydroascorbate + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
 soybean
-

Purification (Commentary)

Purification (Comment) Organism
using Ni2+-agarose affinity chromatography and gel filtration after reconstitution with hemin Glycine max

Renatured (Commentary)

Renatured (Comment) Organism
the apo-recombinant protein is easily converted to the holo-recombinant form by in vitro reconstitution with hemin Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
root nodules Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 effects of media amendments Glycine max
3 4 affinity purified preparation Glycine max
31.7
-
 purified recombinant enzyme Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-di-(3-ethyl-benzothiazoline-(6)-sulfonic acid) + H2O2 3% relative activity to L-ascorbate Glycine max ? + H2O
-
 ?
guaiacol + H2O2 30.5% activity relative to L-ascorbate Glycine max ?
-
 ?
L-ascorbate + H2O2
-
 Glycine max dehydroascorbate + H2O
-
 ?
L-ascorbate + H2O2 physiological role of the enzyme: removal of H2O2, prevention of H2O2 accumulation Glycine max dehydroascorbate + 2 H2O
-
 ?
pyrogallol + H2O2 723% activity relative to L-ascorbate Glycine max 3-hydroxybenzo-1,2-quinone + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9 7
-
 Glycine max
7
-
 assay at Glycine max