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Literature summary for 1.11.1.11 extracted from
- Ascorbate peroxidase from rice seedlings: properties of enzyme isoforms, effects of stresses and protective roles of osmolytes (2004), Plant Sci., 167, 541-550.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,3'-dithiobis(6-nitrobenzoic acid) | 5 mM, 80% residual activity, APX 1, 24% residual activity, APX 2 | Oryza sativa |  |
| Al3+ | inhibition in the range of 0.01-0.5 mM | Oryza sativa | |
| KCN | 1 mM, 69% residual activity, APX 1, 20% residual activity, APX 2 | Oryza sativa | |
| additional information | not inhibitory: alpha,alphaย-dipyridyl, EDTA | Oryza sativa | |
| Na2HAsO4 | inhibition in the range of 0.01-0.5 mM | Oryza sativa | |
| Ni2+ | below 0.01 mM, activation, inhibition above | Oryza sativa | |
| Sodium azide | 1 mM, 72% residual activity, APX 1, 55% residual activity, APX 2 | Oryza sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
H2O2 | pH 6.5, APX 1 | Oryza sativa | |
| 0.076 | - |
H2O2 | pH 7.0, APX 2 | Oryza sativa | |
| 0.2 | - |
ascorbate | pH 7.0, APX 2 | Oryza sativa | |
| 0.4 | - |
L-ascorbate | pH 6.5, APX 1 | Oryza sativa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ni2+ | below 0.01 mM, activation, inhibition above | Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28500 | - |
x * 28500, APX 1, x * 34000, APX 2, SDS-PAGE | Oryza sativa |
| 34000 | - |
x * 28500, APX 1, x * 34000, APX 2, SDS-PAGE | Oryza sativa |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethylene glycol | PEG 6000, 40% in assay medium, 50% inhibition of aPX 1, 65% inhibition of APX 2 with increase in enzyme Km values, incorporation of 1 M proline, glycine betaine or sucrose in presence of PEG in the assay medium restores | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
isoforms APX 1, APX 2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| both APX 1 and APX 2 | Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Oryza sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + H2O2 | 30% of the activity with ascorbate, APX 1, 13% of the activity with ascorbate, APX 2 | Oryza sativa | ? + H2O | - |
? | |
| guaiacol + H2O2 | 45% of the activity with ascorbate, APX 1, 15% of the activity with ascorbate, APX 2 | Oryza sativa | ? + H2O | - |
? | |
| L-ascorbate + H2O2 | - |
Oryza sativa | dehydroascorbate + H2O | - |
? | |
| NADH + H+ + H2O2 | 10% of the activity with ascorbate, APX 1, 1% of the activity with ascorbate, APX 2 | Oryza sativa | NAD+ + H2O | - |
? | |
| NADPH + H+ + H2O2 | 27% of the activity with ascorbate, APX 1, 21% of the activity with ascorbate, APX 2 | Oryza sativa | NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28500, APX 1, x * 34000, APX 2, SDS-PAGE | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| APX 1 | - |
Oryza sativa |
| APX 2 | - |
Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
APX 1 | Oryza sativa |
| 7 | - |
APX 2 | Oryza sativa |
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