Cloned (Comment) | Organism |
---|---|
gene AgAPX1, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3), recombinant GFP-tagged enzyme expression in onion epidermal cells for analysis of subcellular localization of AgAPX1. Overexpression of AgAPX1 in Arabidopsis thaliana positively regulates drought tolerance by regulating the stomata aperture. Net photosynthetic rate considerably decreases in the wild-type compared to transgenic lines under drought stress | Apium graveolens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Apium graveolens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | in the heme group | Apium graveolens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-ascorbate + H2O2 + 2 H+ | Apium graveolens | - |
L-ascorbate + L-dehydroascorbate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apium graveolens | - |
cv. Jinnanshiqin | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | Apium graveolens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Apium graveolens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-ascorbate + H2O2 + 2 H+ | - |
Apium graveolens | L-ascorbate + L-dehydroascorbate + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33160, recombinant enzyme, sequence calculation, x * 34000, recombinant enzyme, SDS-PAGE | Apium graveolens |
Synonyms | Comment | Organism |
---|---|---|
ascorbate peroxidase | - |
Apium graveolens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
recombinant enzyme | Apium graveolens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 85 | activity range, profile overview. Inactivation at 90°C, maximum activity at 55°C | Apium graveolens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant enzyme | Apium graveolens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | activity range, profile overview | Apium graveolens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Apium graveolens |
General Information | Comment | Organism |
---|---|---|
evolution | sequence comparison, phylogenetic analysis and tree | Apium graveolens |
physiological function | recombinant overexpression of celery enzyme AgAPX1 in Arabidopsis thaliana positively regulates drought tolerance by regulating the stomata aperture, physiological changes in Arabidopsis leaves exposed to drought stress, phenotype, overview. The AgAPX1 gene seems to be involved in the response of celery to drought stress. The response of the AgAPX1 gene to adversity may be attributed to the conservation of APX sequences among different species | Apium graveolens |