Cloned (Comment) | Organism |
---|---|
the baculovirus expression vector system, using the Autographa californica nucleopolyhedrovirus and the Sf9 insect cell line, is used to produce recombinant Bi-PHGPx proteins, the cDNA fragment containing the full-length open reading frame is inserted into the transfer vector pBAC1 | Bombus ignitus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002353 | - |
phosphatidylcholine hydroperoxide | apparent KM | Bombus ignitus | |
0.0008656 | - |
H2O2 | apparent KM | Bombus ignitus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenium | - |
Bombus ignitus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
determined by SDS-PAGE | Bombus ignitus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + a lipid hydroperoxide | Bombus ignitus | - |
glutathione disulfide + lipid + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombus ignitus | C3VVL8 | - |
- |
Purification (Comment) | Organism |
---|---|
using a HisTrap column | Bombus ignitus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermis | - |
Bombus ignitus | - |
fat body | extraction of genomic DNA | Bombus ignitus | - |
hemolymph | - |
Bombus ignitus | - |
midgut | - |
Bombus ignitus | - |
muscle | - |
Bombus ignitus | - |
whole body | cDNA library | Bombus ignitus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + a lipid hydroperoxide | - |
Bombus ignitus | glutathione disulfide + lipid + H2O | - |
? | |
glutathione + H2O2 | activity assay | Bombus ignitus | ? | - |
? | |
glutathione + phosphatidylcholine hydroperoxide | activity assay | Bombus ignitus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bi-PHGPx | - |
Bombus ignitus |
GPx4 | - |
Bombus ignitus |
PHGPX | - |
Bombus ignitus |
phospholipid-hydroperoxide glutathione peroxidase | - |
Bombus ignitus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Bombus ignitus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
thermal stability shows that the activity is significantly decreased when the temperature is higher than 40°C | Bombus ignitus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Bombus ignitus |
7.5 | - |
activity assay | Bombus ignitus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | - |
Bombus ignitus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glutathione | - |
Bombus ignitus |
Organism | Comment | Expression |
---|---|---|
Bombus ignitus | Bi-PHGPx transcripts are up-regulated by stresses, such as wounding, H2O2 exposure, external temperature shock, and starvation | up |