Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-hydroxy acid | stimulates by chelating Mn3+ and stabilizing its high redox potential | Phanerodontia chrysosporium | |
citrate | stimulates by chelating Mn3+ and stabilizing its high redox potential | Phanerodontia chrysosporium | |
H2O2 | H2O2-dependent | Phanerodontia chrysosporium | |
Lactate | stimulates by complexing with and stabilizing Mn3+ | Phanerodontia chrysosporium | |
succinate | activates, stabilizes Mn3+ less effective than citrate or lactate | Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
- |
Phanerodontia chrysosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | important component of lignin degradation system | Mn3+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
white rot basidomycete | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Phanerodontia chrysosporium |
Purification (Comment) | Organism |
---|---|
- |
Phanerodontia chrysosporium |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O | kinetic mechanism | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Mn2+ is an obligatory substrate for MnP compound II, whereas compound I formation occurs with Mn2+, p-cresol and organic peroxides, e.g. peracetic acid, m-chloroperoxybenzoic acid and p-nitroperoxybenzoic acid | Phanerodontia chrysosporium | Mn3+ + H2O | alpha-hydroxy acids, e.g. lactate, facilitate the dissociation of Mn3+ from enzyme | ? | |
Mn2+ + H+ + H2O2 | Mn2+ is an obligatory substrate for MnP compound II, whereas compound I formation occurs with Mn2+, p-cresol and organic peroxides, e.g. peracetic acid, m-chloroperoxybenzoic acid and p-nitroperoxybenzoic acid | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes phenolic lignin model compounds | ? | |
Mn2+ + H+ + H2O2 | Mn2+ is an obligatory substrate for MnP compound II, whereas compound I formation occurs with Mn2+, p-cresol and organic peroxides, e.g. peracetic acid, m-chloroperoxybenzoic acid and p-nitroperoxybenzoic acid | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ acts as obligatory redox coupler, oxidizing various phenols, dyes and amines | ? | |
Mn2+ + H+ + H2O2 | Mn2+ is an obligatory substrate for MnP compound II, whereas compound I formation occurs with Mn2+, p-cresol and organic peroxides, e.g. peracetic acid, m-chloroperoxybenzoic acid and p-nitroperoxybenzoic acid | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes p-cresol | ? | |
Mn2+ + H+ + H2O2 | Mn2+ is an obligatory substrate for MnP compound II, whereas compound I formation occurs with Mn2+, p-cresol and organic peroxides, e.g. peracetic acid, m-chloroperoxybenzoic acid and p-nitroperoxybenzoic acid | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes amines | ? | |
Mn2+ + H+ + H2O2 | Mn2+ is an obligatory substrate for MnP compound II, whereas compound I formation occurs with Mn2+, p-cresol and organic peroxides, e.g. peracetic acid, m-chloroperoxybenzoic acid and p-nitroperoxybenzoic acid | Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes a variety of phenols | ? | |
Mn2+ + H+ + H2O2 | Mn2+ is an obligatory substrate for MnP compound II, whereas compound I formation occurs with Mn2+, p-cresol and organic peroxides, e.g. peracetic acid, m-chloroperoxybenzoic acid and p-nitroperoxybenzoic acid | Phanerodontia chrysosporium | Mn3+ + H2O | chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential | ? | |
Mn2+ + H+ + H2O2 | important component of lignin degradation system | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
additional information | catalytic cycle of enzyme, oxidation states: native enzyme via compound I via compound II to native enzyme, Mn2+ and phenols reduce MnP compound I to compound II, but only Mn2+ is a substrate for MnP compound II, Mn(II)/Mn(III) redox couple enables enzyme to rapidly oxidize terminal phenolic substrates | Phanerodontia chrysosporium | ? | - |
? | |
additional information | primary reaction product of peroxidation with H2O2 is enzyme compound I, formation of compound II from I follows second-order kinetic | Phanerodontia chrysosporium | ? | - |
? | |
additional information | enzyme oxidizes phenol red | Phanerodontia chrysosporium | ? | - |
? | |
additional information | in presence of Mn2+ enzyme oxidizes various organic compounds | Phanerodontia chrysosporium | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Phanerodontia chrysosporium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.1 | 8.3 | in presence of H2O2 the formation of enzyme compound I is independent of pH over the range | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | one iron protoporphyrin IX prosthetic group per enzyme molecule | Phanerodontia chrysosporium |