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Literature summary for 1.11.1.13 extracted from
- Engineering of the H2O2-binding pocket region of a recombinant manganese peroxidase to be resistant to H2O2 (2001), FEBS Lett., 509, 111-114.
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | mediated system of degradation is potentially valuable for degradation of synthetic polymers and of environmental pollutants | Phanerodontia chrysosporium |
| paper production | mediated system of degradation is potentially valuable for pulp and paper industries | Phanerodontia chrysosporium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA encoding MnP2 is cloned and expressed in Escherichia coli BL21(DE3)LysS | Phanerodontia chrysosporium |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure | Phanerodontia chrysosporium |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| M237L | engineered mutant | Phanerodontia chrysosporium |
| M273L | mutant with high H2O2 resistance, i.e. 4.1fold higher than that of wild-type, Met-273 is located near the active site pocket and is converted to a non-oxidizable Leu | Phanerodontia chrysosporium |
| M67L | engineered mutant | Phanerodontia chrysosporium |
| N81S | mutant enzyme is not inhibited by 1 mM H2O2, H2O2-dependency is 5.5fold higher than that of wild-type, engineering of Asn-81, which might have conformational changes due to the environment of the pocket, to a non-bulky and non-oxidizable Ser | Phanerodontia chrysosporium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | 1 mM, complete, irreversible inactivation of wild-type enzyme correlated with the production of methionine sulfoxide, full activity can be retained by engineering Asn-81, which might have conformational changes due to the environment of the pocket, to a non-bulky and non-oxidizable Ser | Phanerodontia chrysosporium |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | Q02567 | MnP2 | - |
| Phanerodontia chrysosporium | Q02567 | strain ATCC 64314 | - |
| Phanerodontia chrysosporium MnP2 | Q02567 | MnP2 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | native MnP is glycosylated, but not recombinant MnP | Phanerodontia chrysosporium |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of recombinant MnP, expressed in Escherichia coli | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium MnP2 | Mn3+ + H2O | - |
? | |
| additional information | structural properties | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | structural properties | Phanerodontia chrysosporium MnP2 | ? | - |
? | |
| additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Phanerodontia chrysosporium MnP2 | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
very sensitive to thermal treatment | Phanerodontia chrysosporium |
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