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Literature summary for 1.11.1.13 extracted from
- Purification and characterization of a manganese peroxidases from the litter-decomposing basidiomycetes Agrocybe praecox and Stropharia coronilla (2002), Enzyme Microb. Technol., 30, 550-555.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
H2O2 | 0.1 mM Mn2+, MnP1 | Agrocybe praecox |  |
| 0.002 | - |
H2O2 | 0.1 mM Mn2+, MnP1 | Stropharia coronilla | |
| 0.012 | - |
Mn2+ | 0.1 mM H2O2, MnP1 | Stropharia coronilla | |
| 0.017 | - |
Mn2+ | 0.1 mM H2O2, MnP1 | Agrocybe praecox | |
| 0.667 | - |
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) | 0.1 mM H2O2, Mn2+-independent activity, MnP1 | Agrocybe praecox | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Agrocybe praecox | - |
- |
| extracellular | - |
Stropharia coronilla | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | MnP is induced by Mn2+ | Agrocybe praecox | |
| additional information | MnP is induced by Mn2+ | Stropharia coronilla |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
x * 41000, MnP1 and MnP2, x * 43000, MnP3, SDS-PAGE | Stropharia coronilla |
| 42000 | - |
x * 42000, SDS-PAGE | Agrocybe praecox |
| 43000 | - |
x * 41000, MnP1 and MnP2, x * 43000, MnP3, SDS-PAGE | Stropharia coronilla |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Mn2+ + H+ + H2O2 | Agrocybe praecox | important component of lignin degradation system | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | Stropharia coronilla | important component of lignin degradation system | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | Stropharia coronilla TM 47-1 | important component of lignin degradation system | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | Agrocybe praecox TM 70.84 | important component of lignin degradation system | Mn3+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrocybe praecox | - |
2 isoenzymes | - |
| Agrocybe praecox TM 70.84 | - |
2 isoenzymes | - |
| Stropharia coronilla | - |
3 isoenzymes: Mn2+-inducible MnP1 and MnP3, partly constitutive MnP2 | - |
| Stropharia coronilla TM 47-1 | - |
3 isoenzymes: Mn2+-inducible MnP1 and MnP3, partly constitutive MnP2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification | Agrocybe praecox |
| partial purification, MnP2 and MnP3 are not separated | Stropharia coronilla |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 128 | - |
- |
Agrocybe praecox |
| 692 | - |
- |
Stropharia coronilla |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 + H+ | - |
Agrocybe praecox | ? | - |
r | |
| 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 + H+ | - |
Agrocybe praecox TM 70.84 | ? | - |
r | |
| Mn2+ + H+ + H2O2 | - |
Agrocybe praecox | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | - |
Stropharia coronilla | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | important component of lignin degradation system | Agrocybe praecox | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | important component of lignin degradation system | Stropharia coronilla | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | - |
Stropharia coronilla TM 47-1 | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | important component of lignin degradation system | Stropharia coronilla TM 47-1 | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | - |
Agrocybe praecox TM 70.84 | Mn3+ + H2O | - |
? | |
| Mn2+ + H+ + H2O2 | important component of lignin degradation system | Agrocybe praecox TM 70.84 | Mn3+ + H2O | - |
? | |
| additional information | in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Agrocybe praecox | ? | - |
? | |
| additional information | in absence of Mn2+ the enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Agrocybe praecox TM 70.84 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE | Agrocybe praecox |
| ? | x * 41000, MnP1 and MnP2, x * 43000, MnP3, SDS-PAGE | Stropharia coronilla |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Agrocybe praecox |
| 25 | - |
assay at | Stropharia coronilla |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
MnP1: pI 6.3-7.1, MnP2: 3.5-3.7, MnP3: 5.1 | Stropharia coronilla |
| additional information | - |
2 isoenzymes: pI 6.3-7.0 | Agrocybe praecox |
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