Application | Comment | Organism |
---|---|---|
paper production | - |
Pleurotus ostreatus |
paper production | - |
Pleurotus eryngii |
paper production | bleaching of paper pulp | Phanerodontia chrysosporium |
paper production | bleaching of paper pulp | Bjerkandera sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | additional information | Pleurotus ostreatus | |
additional information | - |
additional information | additional information | Phanerodontia chrysosporium | |
additional information | - |
additional information | additional information | Bjerkandera adusta | |
additional information | - |
additional information | additional information | Pleurotus pulmonarius | |
additional information | - |
additional information | additional information | Pleurotus eryngii | |
additional information | - |
additional information | additional information | Bjerkandera sp. | |
0.0031 | - |
H2O2 | 0.1 mM Mn2+, BOS1 | Bjerkandera sp. | |
0.0047 | - |
H2O2 | 0.1 mM Mn2+, BOS2 | Bjerkandera sp. | |
0.005 | - |
H2O2 | 0.1 mM Mn2+, PCH6 | Phanerodontia chrysosporium | |
0.006 | - |
H2O2 | 0.1 mM Mn2+, PCH5 | Phanerodontia chrysosporium | |
0.0066 | - |
2,6-dimethoxyphenol | Mn2+-dependent activity, PCH6 | Phanerodontia chrysosporium | |
0.0092 | - |
H2O2 | 0.1 mM Mn2+, PCH4 | Phanerodontia chrysosporium | |
0.011 | - |
2,6-dimethoxyphenol | Mn2+-dependent activity, BOS2 | Bjerkandera sp. | |
0.011 | - |
2,6-dimethoxyphenol | Mn2+-dependent activity, PCH5 | Phanerodontia chrysosporium | |
0.011 | - |
2,6-dimethoxyphenol | isoform BOS2, at pH 5.0 and 30°C | Bjerkandera sp. | |
0.0156 | - |
Mn2+ | PCH4 | Phanerodontia chrysosporium | |
0.0165 | - |
Mn2+ | BOS1 | Bjerkandera sp. | |
0.0175 | - |
Mn2+ | PCH5 | Phanerodontia chrysosporium | |
0.018 | - |
2,6-dimethoxyphenol | Mn2+-dependent activity, PCH4 | Phanerodontia chrysosporium | |
0.0195 | - |
2,6-dimethoxyphenol | Mn2+-dependent activity, BOS1 | Bjerkandera sp. | |
0.0195 | - |
2,6-dimethoxyphenol | isoform BOS1, at pH 5.0 and 30°C | Bjerkandera sp. | |
0.0254 | - |
Mn2+ | BOS2 | Bjerkandera sp. | |
0.045 | - |
Mn2+ | PCH6 | Phanerodontia chrysosporium | |
0.106 | - |
2,6-dimethoxyphenol | Mn2+-independent activity, BOS2 | Bjerkandera sp. | |
0.106 | - |
2,6-dimethoxyphenol | isoform BOS2, at pH 5.0 and 30°C | Bjerkandera sp. | |
0.121 | - |
2,6-dimethoxyphenol | Mn2+-independent activity, BOS1 | Bjerkandera sp. | |
0.121 | - |
2,6-dimethoxyphenol | isoform BOS1, at pH 5.0 and 30°C | Bjerkandera sp. | |
3.2 | - |
veratryl alcohol | BOS1 | Bjerkandera sp. | |
3.2 | - |
veratryl alcohol | isoform BOS1, at pH 5.0 and 30°C | Bjerkandera sp. | |
5.33 | - |
veratryl alcohol | BOS2 | Bjerkandera sp. | |
5.33 | - |
veratryl alcohol | isoform BOS2, at pH 5.0 and 30°C | Bjerkandera sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
extracellular | - |
Bjerkandera adusta | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
around 40 kDa, gel filtration | Phanerodontia chrysosporium |
40000 | - |
around 40 kDa, gel filtration | Bjerkandera sp. |
43000 | - |
1 * 45000, PCH4 and PCH6, 1 * 43000, PCH5, SDS-PAGE | Phanerodontia chrysosporium |
44000 | - |
1 * 45000, BOS1, 1 * 44000, BOS2, SDS-PAGE | Bjerkandera sp. |
45000 | - |
1 * 45000, PCH4 and PCH6, 1 * 43000, PCH5, SDS-PAGE | Phanerodontia chrysosporium |
45000 | - |
1 * 45000, BOS1, 1 * 44000, BOS2, SDS-PAGE | Bjerkandera sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Pleurotus ostreatus | involved in lignin-degradation | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | involved in lignin-degradation | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Pleurotus eryngii | involved in lignin-degradation | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium VKM F-1767 | involved in lignin-degradation | Mn3+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bjerkandera adusta | - |
- |
- |
Bjerkandera sp. | - |
2 isoenzymes: BOS1 and BOS2 | - |
Bjerkandera sp. BOS55 | - |
2 isoenzymes: BOS1 and BOS2 | - |
Phanerodontia chrysosporium | - |
- |
- |
Phanerodontia chrysosporium | - |
3 isoenzymes: PCH4, PCH5, PCH6 | - |
Phanerodontia chrysosporium VKM F-1767 | - |
- |
- |
Pleurotus eryngii | - |
- |
- |
Pleurotus ostreatus | - |
- |
- |
Pleurotus pulmonarius | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Pleurotus ostreatus |
glycoprotein | - |
Phanerodontia chrysosporium |
glycoprotein | - |
Bjerkandera adusta |
glycoprotein | - |
Pleurotus eryngii |
glycoprotein | 2 isoenzymes differ in glycosylation degree, anthrone analysis: 5-7% total carbohydrate, SDS-PAGE after Endo-H treatment: 4.5% N-linked carbohydrate content in BOS1 and 2.3% in BOS2 | Bjerkandera sp. |
Purification (Comment) | Organism |
---|---|
2 isoenzymes: BOS1 and BOS2 | Bjerkandera sp. |
3 isozymes | Phanerodontia chrysosporium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pleurotus ostreatus |
additional information | - |
- |
Phanerodontia chrysosporium |
additional information | - |
- |
Bjerkandera adusta |
additional information | - |
- |
Bjerkandera sp. |
Storage Stability | Organism |
---|---|
-20°C, stable | Phanerodontia chrysosporium |
-20°C, stable | Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dimethoxyphenol + H2O2 | - |
Bjerkandera sp. | coerulignone + H2O | - |
r | |
2,6-dimethoxyphenol + Mn2+ + H2O2 | - |
Bjerkandera sp. | coerulignone + Mn3+ + H2O | - |
r | |
2,6-dimethoxyphenol + Mn2+ + H2O2 | - |
Bjerkandera sp. BOS55 | coerulignone + Mn3+ + H2O | - |
r | |
Mn2+ + H+ + H2O2 | - |
Bjerkandera adusta | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus pulmonarius | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus ostreatus | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus eryngii | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | - |
Bjerkandera sp. | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | involved in lignin-degradation | Pleurotus ostreatus | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | involved in lignin-degradation | Phanerodontia chrysosporium | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | involved in lignin-degradation | Pleurotus eryngii | Mn3+ + H2O | - |
? | |
Mn2+ + H+ + H2O2 | - |
Bjerkandera sp. BOS55 | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium VKM F-1767 | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | involved in lignin-degradation | Phanerodontia chrysosporium VKM F-1767 | Mn3+ + H2O | - |
? | |
additional information | Mn-mediated and Mn-independent activity on phenolic and non-phenolic aromatic substrates | Bjerkandera sp. | ? | - |
? | |
additional information | Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol | Pleurotus ostreatus | ? | - |
? | |
additional information | Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol | Phanerodontia chrysosporium | ? | - |
? | |
additional information | Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol | Pleurotus eryngii | ? | - |
? | |
additional information | Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol | Bjerkandera sp. | ? | - |
? | |
additional information | enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ | Phanerodontia chrysosporium | ? | - |
? | |
additional information | no activity with veratryl alcohol | Pleurotus ostreatus | ? | - |
? | |
additional information | no activity with veratryl alcohol | Phanerodontia chrysosporium | ? | - |
? | |
additional information | no activity with veratryl alcohol | Bjerkandera adusta | ? | - |
? | |
additional information | Mn-mediated and Mn-independent activity on phenolic and non-phenolic aromatic substrates | Bjerkandera sp. BOS55 | ? | - |
? | |
additional information | Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol | Bjerkandera sp. BOS55 | ? | - |
? | |
additional information | Mn2+-independent peroxidase activity on 2,6-dimethoxyphenol and veratryl alcohol | Phanerodontia chrysosporium VKM F-1767 | ? | - |
? | |
additional information | enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ | Phanerodontia chrysosporium VKM F-1767 | ? | - |
? | |
additional information | no activity with veratryl alcohol | Phanerodontia chrysosporium VKM F-1767 | ? | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Bjerkandera sp. | ? | - |
r | |
veratryl alcohol + H2O2 + H+ | - |
Bjerkandera sp. BOS55 | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Pleurotus ostreatus |
monomer | 1 * 45000, PCH4 and PCH6, 1 * 43000, PCH5, SDS-PAGE | Phanerodontia chrysosporium |
monomer | 1 * 45000, BOS1, 1 * 44000, BOS2, SDS-PAGE | Bjerkandera sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pleurotus ostreatus |
additional information | - |
- |
Phanerodontia chrysosporium |
additional information | - |
- |
Bjerkandera adusta |
additional information | - |
- |
Pleurotus eryngii |
additional information | - |
BOS1: pI 3.45, BOS2: pI 3.4 | Bjerkandera sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Pleurotus ostreatus | |
heme | heme protein | Phanerodontia chrysosporium | |
heme | heme protein | Bjerkandera adusta | |
heme | heme protein | Pleurotus eryngii |