Application | Comment | Organism |
---|---|---|
degradation | Mn peroxidases are of much interest biotechnologically because of their potentially applications in bioremdeial waste treatment and in catalyzing difficult chemical transfromations | Phanerodontia chrysosporium |
Cloned (Comment) | Organism |
---|---|
into the vector pET-22b, used for in vitro transcription and translation in a wheat germ extract system | Phanerodontia chrysosporium |
Protein Variants | Comment | Organism |
---|---|---|
N131D | mutant displays a similar catalysis pattern to that of wild-type enzyme, Asn131 is the only potential glycosylation site | Phanerodontia chrysosporium |
R42A | mutant displays a similar catalysis pattern to that of wild-type enzyme, Arg42 is forming the peroxide binding pocket | Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Manganese | - |
Phanerodontia chrysosporium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
determined by Western blot analysis | Phanerodontia chrysosporium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Phanerodontia chrysosporium | - |
Mn3+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | Q02567 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.055 U/ml, R42A mutant, in the presence of 2,6-dimethoxyphenol | Phanerodontia chrysosporium |
additional information | - |
0.069 U/ml, N131D mutant, in the presence of 2,6-dimethoxyphenol | Phanerodontia chrysosporium |
additional information | - |
0.076 U/ml, wild-type enzyme, in the presence of 2,6-dimethoxyphenol | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + 2,6-dimethoxyphenol + H2O2 | - |
Phanerodontia chrysosporium | ? | - |
? | |
Mn2+ + H+ + H2O2 | - |
Phanerodontia chrysosporium | Mn3+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
manganese peroxidase | - |
Phanerodontia chrysosporium |
MnP | - |
Phanerodontia chrysosporium |
mnp1 | - |
Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Phanerodontia chrysosporium |